1eg0: Difference between revisions
New page: left|200px<br /><applet load="1eg0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eg0, resolution 11.5Å" /> '''FITTING OF COMPONENT... |
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[[Image:1eg0.gif|left|200px]]<br /><applet load="1eg0" size=" | [[Image:1eg0.gif|left|200px]]<br /><applet load="1eg0" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1eg0, resolution 11.5Å" /> | caption="1eg0, resolution 11.5Å" /> | ||
'''FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME'''<br /> | '''FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME'''<br /> | ||
==Overview== | ==Overview== | ||
Over 73,000 projections of the E. coli ribosome bound with | Over 73,000 projections of the E. coli ribosome bound with formyl-methionyl initiator tRNAf(Met) were used to obtain an 11.5 A cryo-electron microscopy map of the complex. This map allows identification of RNA helices, peripheral proteins, and intersubunit bridges. Comparison of double-stranded RNA regions and positions of proteins identified in both cryo-EM and X-ray maps indicates good overall agreement but points to rearrangements of ribosomal components required for the subunit association. Fitting of known components of the 50S stalk base region into the map defines the architecture of the GTPase-associated center and reveals a major change in the orientation of the alpha-sarcin-ricin loop. Analysis of the bridging connections between the subunits provides insight into the dynamic signaling mechanism between the ribosomal subunits. | ||
==About this Structure== | ==About this Structure== | ||
1EG0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with 4SU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1EG0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=4SU:'>4SU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EG0 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Agrawal, R | [[Category: Agrawal, R K.]] | ||
[[Category: Frank, J.]] | [[Category: Frank, J.]] | ||
[[Category: Gabashvili, I | [[Category: Gabashvili, I S.]] | ||
[[Category: Grassucci, R | [[Category: Grassucci, R A.]] | ||
[[Category: Penczek, P.]] | [[Category: Penczek, P.]] | ||
[[Category: Spahn, C | [[Category: Spahn, C M.T.]] | ||
[[Category: Svergun, D | [[Category: Svergun, D I.]] | ||
[[Category: 4SU]] | [[Category: 4SU]] | ||
[[Category: 70s ribosome]] | [[Category: 70s ribosome]] | ||
[[Category: low resolution model]] | [[Category: low resolution model]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:27:18 2008'' | ||
Revision as of 13:27, 21 February 2008
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FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME
OverviewOverview
Over 73,000 projections of the E. coli ribosome bound with formyl-methionyl initiator tRNAf(Met) were used to obtain an 11.5 A cryo-electron microscopy map of the complex. This map allows identification of RNA helices, peripheral proteins, and intersubunit bridges. Comparison of double-stranded RNA regions and positions of proteins identified in both cryo-EM and X-ray maps indicates good overall agreement but points to rearrangements of ribosomal components required for the subunit association. Fitting of known components of the 50S stalk base region into the map defines the architecture of the GTPase-associated center and reveals a major change in the orientation of the alpha-sarcin-ricin loop. Analysis of the bridging connections between the subunits provides insight into the dynamic signaling mechanism between the ribosomal subunits.
About this StructureAbout this Structure
1EG0 is a Protein complex structure of sequences from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Solution structure of the E. coli 70S ribosome at 11.5 A resolution., Gabashvili IS, Agrawal RK, Spahn CM, Grassucci RA, Svergun DI, Frank J, Penczek P, Cell. 2000 Mar 3;100(5):537-49. PMID:10721991
Page seeded by OCA on Thu Feb 21 12:27:18 2008