1war: Difference between revisions
New page: left|200px<br /> <applet load="1war" size="450" color="white" frame="true" align="right" spinBox="true" caption="1war, resolution 2.22Å" /> '''RECOMBINANT HUMAN P... |
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==About this Structure== | ==About this Structure== | ||
1WAR is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with NAG, FE and PO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WAR OCA]]. | 1WAR is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with NAG, FE and PO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WAR OCA]]. | ||
==Reference== | ==Reference== | ||
Crystal structures of recombinant human purple Acid phosphatase with and without an inhibitory conformation of the repression loop., Strater N, Jasper B, Scholte M, Krebs B, Duff AP, Langley DB, Han R, Averill BA, Freeman HC, Guss JM, J Mol Biol. 2005 Aug 5;351(1):233-46. Epub 2005 Apr 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15993892 15993892] | Crystal structures of recombinant human purple Acid phosphatase with and without an inhibitory conformation of the repression loop., Strater N, Jasper B, Scholte M, Krebs B, Duff AP, Langley DB, Han R, Averill BA, Freeman HC, Guss JM, J Mol Biol. 2005 Aug 5;351(1):233-46. Epub 2005 Apr 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15993892 15993892] | ||
[[Category: Acid phosphatase]] | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: uteroferrin]] | [[Category: uteroferrin]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:56:22 2007'' | ||
Revision as of 14:51, 30 October 2007
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RECOMBINANT HUMAN PURPLE ACID PHOSPHATASE EXPRESSED IN PICHIA PASTORIS
OverviewOverview
The crystal structure of human purple acid phosphatase recombinantly, expressed in Escherichia coli (rHPAP(Ec)) and Pichia pastoris (rHPAP(Pp)), has been determined in two different crystal forms, both at 2.2A, resolution. In both cases, the enzyme crystallized in its oxidized, (inactive) state, in which both Fe atoms in the dinuclear active site are, Fe(III). The main difference between the two structures is the, conformation of the enzyme "repression loop". Proteolytic cleavage of this, loop in vivo or in vitro results in significant activation of the, mammalian PAPs. In the crystals obtained from rHPAP(Ec), the carboxylate, side-chain of Asp145 of this loop acts as a bidentate ligand that bridges, the two metal atoms, in a manner analogous to a possible binding mode for, a phosphate ... [(full description)]
About this StructureAbout this Structure
1WAR is a [Single protein] structure of sequence from [Homo sapiens] with NAG, FE and PO4 as [ligands]. Active as [Acid phosphatase], with EC number [3.1.3.2]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Crystal structures of recombinant human purple Acid phosphatase with and without an inhibitory conformation of the repression loop., Strater N, Jasper B, Scholte M, Krebs B, Duff AP, Langley DB, Han R, Averill BA, Freeman HC, Guss JM, J Mol Biol. 2005 Aug 5;351(1):233-46. Epub 2005 Apr 26. PMID:15993892
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