1ogb: Difference between revisions
New page: left|200px<br /> <applet load="1ogb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ogb, resolution 1.85Å" /> '''CHITINASE B FROM SE... |
No edit summary |
||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
1OGB is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]] with SO4 and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OGB OCA]]. | 1OGB is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]] with SO4 and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Chitinase Chitinase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OGB OCA]]. | ||
==Reference== | ==Reference== | ||
Structure of the D142N mutant of the family 18 chitinase ChiB from Serratia marcescens and its complex with allosamidin., Vaaje-Kolstad G, Houston DR, Rao FV, Peter MG, Synstad B, van Aalten DM, Eijsink VG, Biochim Biophys Acta. 2004 Jan 14;1696(1):103-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14726210 14726210] | Structure of the D142N mutant of the family 18 chitinase ChiB from Serratia marcescens and its complex with allosamidin., Vaaje-Kolstad G, Houston DR, Rao FV, Peter MG, Synstad B, van Aalten DM, Eijsink VG, Biochim Biophys Acta. 2004 Jan 14;1696(1):103-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14726210 14726210] | ||
[[Category: Chitinase]] | |||
[[Category: Serratia marcescens]] | [[Category: Serratia marcescens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 27: | Line 28: | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:54:20 2007'' | ||
Revision as of 14:49, 30 October 2007
|
CHITINASE B FROM SERRATIA MARCESCENS MUTANT D142N
OverviewOverview
Catalysis by ChiB, a family 18 chitinase from Serratia marcescens, involves a conformational change of Asp142 which is part of a, characteristic D(140)XD(142)XE(144) sequence motif. In the free enzyme, Asp142 points towards Asp140, whereas it rotates towards the catalytic, acid, Glu144, upon ligand binding. Mutation of Asp142 to Asn reduced, k(cat) and affinity for allosamidin, a competitive inhibitor. The X-ray, structure of the D142N mutant showed that Asn142 points towards Glu144 in, the absence of a ligand. The active site also showed other structural, adjustments (Tyr10, Ser93) that had previously been observed in the, wild-type enzyme upon substrate binding. The X-ray structure of a complex, of D142N with allosamidin, a pseudotrisaccharide competitive inhibitor, was essentially ... [(full description)]
About this StructureAbout this Structure
1OGB is a [Single protein] structure of sequence from [Serratia marcescens] with SO4 and GOL as [ligands]. Active as [Chitinase], with EC number [3.2.1.14]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Structure of the D142N mutant of the family 18 chitinase ChiB from Serratia marcescens and its complex with allosamidin., Vaaje-Kolstad G, Houston DR, Rao FV, Peter MG, Synstad B, van Aalten DM, Eijsink VG, Biochim Biophys Acta. 2004 Jan 14;1696(1):103-11. PMID:14726210
Page seeded by OCA on Tue Oct 30 13:54:20 2007