1dxw: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
Few structures of viral serine proteases, those encoded by the Sindbis and | Few structures of viral serine proteases, those encoded by the Sindbis and Semliki Forest viruses, hepatitis C virus (HCV) and cytomegalovirus, have been reported. In the life cycle of HCV a crucial role is played by a chymotrypsin-like serine protease encoded at the N-terminus of the viral NS3 protein, the solution structure of which we present here complexed with a covalently bound reversible inhibitor. Unexpectedly, the residue in the P2 position of the inhibitor induces an effective stabilization of the catalytic His-Asp hydrogen bond, by shielding that region of the protease from the solvent. This interaction appears crucial in the activation of the enzyme catalytic machinery and represents an unprecedented observation for this family of enzymes. Our data suggest that natural substrates of this serine protease could contribute to the enzyme activation by a similar induced-fit mechanism. The high degree of similarity at the His-Asp catalytic site region between HCV NS3 and other viral serine proteases suggests that this behaviour could be a more general feature for this category of viral enzymes. | ||
==About this Structure== | ==About this Structure== | ||
| Line 15: | Line 15: | ||
[[Category: Barbato, G.]] | [[Category: Barbato, G.]] | ||
[[Category: Bazzo, R.]] | [[Category: Bazzo, R.]] | ||
[[Category: Cicero, D | [[Category: Cicero, D O.]] | ||
[[Category: Cordier, F.]] | [[Category: Cordier, F.]] | ||
[[Category: Defrancesco, R.]] | [[Category: Defrancesco, R.]] | ||
[[Category: Gerlach, B.]] | [[Category: Gerlach, B.]] | ||
[[Category: Grzesiek, S.]] | [[Category: Grzesiek, S.]] | ||
[[Category: Matassa, V | [[Category: Matassa, V G.]] | ||
[[Category: Narjes, F.]] | [[Category: Narjes, F.]] | ||
[[Category: Sambucini, S.]] | [[Category: Sambucini, S.]] | ||
| Line 29: | Line 29: | ||
[[Category: serine protease]] | [[Category: serine protease]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:21:40 2008'' | ||
Revision as of 13:21, 21 February 2008
|
STRUCTURE OF HETERO COMPLEX OF NON STRUCTURAL PROTEIN (NS) OF HEPATITIS C VIRUS (HCV) AND SYNTHETIC PEPTIDIC COMPOUND
OverviewOverview
Few structures of viral serine proteases, those encoded by the Sindbis and Semliki Forest viruses, hepatitis C virus (HCV) and cytomegalovirus, have been reported. In the life cycle of HCV a crucial role is played by a chymotrypsin-like serine protease encoded at the N-terminus of the viral NS3 protein, the solution structure of which we present here complexed with a covalently bound reversible inhibitor. Unexpectedly, the residue in the P2 position of the inhibitor induces an effective stabilization of the catalytic His-Asp hydrogen bond, by shielding that region of the protease from the solvent. This interaction appears crucial in the activation of the enzyme catalytic machinery and represents an unprecedented observation for this family of enzymes. Our data suggest that natural substrates of this serine protease could contribute to the enzyme activation by a similar induced-fit mechanism. The high degree of similarity at the His-Asp catalytic site region between HCV NS3 and other viral serine proteases suggests that this behaviour could be a more general feature for this category of viral enzymes.
About this StructureAbout this Structure
1DXW is a Single protein structure of sequence from Viruses with as ligand. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
ReferenceReference
Inhibitor binding induces active site stabilization of the HCV NS3 protein serine protease domain., Barbato G, Cicero DO, Cordier F, Narjes F, Gerlach B, Sambucini S, Grzesiek S, Matassa VG, De Francesco R, Bazzo R, EMBO J. 2000 Mar 15;19(6):1195-206. PMID:10716920
Page seeded by OCA on Thu Feb 21 12:21:40 2008