2blr: Difference between revisions
New page: left|200px<br /> <applet load="2blr" size="450" color="white" frame="true" align="right" spinBox="true" caption="2blr, resolution 1.40Å" /> '''THAUMATIN BEFORE A ... |
No edit summary |
||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
2BLR is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]] with TLA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BLR OCA]]. | 2BLR is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]] with TLA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BLR OCA]]. | ||
==Reference== | ==Reference== | ||
| Line 24: | Line 24: | ||
[[Category: taste-modifying protein]] | [[Category: taste-modifying protein]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:52:31 2007'' | ||
Revision as of 14:47, 30 October 2007
|
THAUMATIN BEFORE A HIGH DOSE X-RAY "BURN"
OverviewOverview
Specific radiation damage can be used to solve macromolecular structures, using the radiation-damage-induced phasing (RIP) method. The method has, been investigated for six disulfide-containing test structures (elastase, insulin, lysozyme, ribonuclease A, trypsin and thaumatin) using data sets, that were collected on a third-generation synchrotron undulator beamline, with a highly attenuated beam. Each crystal was exposed to the, unattenuated X-ray beam between the collection of a 'before' and an, 'after' data set. The X-ray 'burn'-induced intensity differences ranged, from 5 to 15%, depending on the protein investigated. X-ray-susceptible, substructures were determined using the integrated direct and Patterson, methods in SHELXD. The best substructures were found by downscaling the, ... [(full description)]
About this StructureAbout this Structure
2BLR is a [Single protein] structure of sequence from [Thaumatococcus daniellii] with TLA as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Improving radiation-damage substructures for RIP., Nanao MH, Sheldrick GM, Ravelli RB, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1227-37. Epub 2005, Aug 16. PMID:16131756
Page seeded by OCA on Tue Oct 30 13:52:31 2007