1dl6: Difference between revisions
New page: left|200px<br /> <applet load="1dl6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dl6" /> '''SOLUTION STRUCTURE OF HUMAN TFIIB N-TERMINA... |
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[[Image:1dl6.gif|left|200px]]<br /> | [[Image:1dl6.gif|left|200px]]<br /><applet load="1dl6" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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'''SOLUTION STRUCTURE OF HUMAN TFIIB N-TERMINAL DOMAIN'''<br /> | '''SOLUTION STRUCTURE OF HUMAN TFIIB N-TERMINAL DOMAIN'''<br /> | ||
==Overview== | ==Overview== | ||
Transcription factor IIB (TFIIB) is an essential component in the | Transcription factor IIB (TFIIB) is an essential component in the formation of the transcription initiation complex in eucaryal and archaeal transcription. TFIIB interacts with a promoter complex containing the TATA-binding protein (TBP) to facilitate interaction with RNA polymerase II (RNA pol II) and the associated transcription factor IIF (TFIIF). TFIIB contains a zinc-binding motif near the N-terminus that is directly involved in the interaction with RNA pol II/TFIIF and plays a crucial role in selecting the transcription initiation site. The solution structure of the N-terminal residues 2-59 of human TFIIB was determined by multidimensional NMR spectroscopy. The structure consists of a nearly tetrahedral Zn(Cys)3(His)1 site confined by type I and "rubredoxin" turns, three antiparallel beta-strands, and disordered loops. The structure is similar to the reported zinc-ribbon motifs in several transcription-related proteins from archaea and eucarya, including Pyrococcus furiosus transcription factor B (PfTFB), human and yeast transcription factor IIS (TFIIS), and Thermococcus celer RNA polymerase II subunit M (TcRPOM). The zinc-ribbon structure of TFIIB, in conjunction with the biochemical analyses, suggests that residues on the beta-sheet are involved in the interaction with RNA pol II/TFIIF, while the zinc-binding site may increase the stability of the beta-sheet. | ||
==About this Structure== | ==About this Structure== | ||
1DL6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1DL6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DL6 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Chen, H | [[Category: Chen, H T.]] | ||
[[Category: Glushka, J.]] | [[Category: Glushka, J.]] | ||
[[Category: Legault, P.]] | [[Category: Legault, P.]] | ||
[[Category: Omichinski, J | [[Category: Omichinski, J G.]] | ||
[[Category: Scott, R | [[Category: Scott, R A.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: zinc ribbon]] | [[Category: zinc ribbon]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:17:45 2008'' | ||
Revision as of 13:17, 21 February 2008
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SOLUTION STRUCTURE OF HUMAN TFIIB N-TERMINAL DOMAIN
OverviewOverview
Transcription factor IIB (TFIIB) is an essential component in the formation of the transcription initiation complex in eucaryal and archaeal transcription. TFIIB interacts with a promoter complex containing the TATA-binding protein (TBP) to facilitate interaction with RNA polymerase II (RNA pol II) and the associated transcription factor IIF (TFIIF). TFIIB contains a zinc-binding motif near the N-terminus that is directly involved in the interaction with RNA pol II/TFIIF and plays a crucial role in selecting the transcription initiation site. The solution structure of the N-terminal residues 2-59 of human TFIIB was determined by multidimensional NMR spectroscopy. The structure consists of a nearly tetrahedral Zn(Cys)3(His)1 site confined by type I and "rubredoxin" turns, three antiparallel beta-strands, and disordered loops. The structure is similar to the reported zinc-ribbon motifs in several transcription-related proteins from archaea and eucarya, including Pyrococcus furiosus transcription factor B (PfTFB), human and yeast transcription factor IIS (TFIIS), and Thermococcus celer RNA polymerase II subunit M (TcRPOM). The zinc-ribbon structure of TFIIB, in conjunction with the biochemical analyses, suggests that residues on the beta-sheet are involved in the interaction with RNA pol II/TFIIF, while the zinc-binding site may increase the stability of the beta-sheet.
About this StructureAbout this Structure
1DL6 is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Structure of a (Cys3His) zinc ribbon, a ubiquitous motif in archaeal and eucaryal transcription., Chen HT, Legault P, Glushka J, Omichinski JG, Scott RA, Protein Sci. 2000 Sep;9(9):1743-52. PMID:11045620
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