1dfj: Difference between revisions
New page: left|200px<br /><applet load="1dfj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dfj, resolution 2.5Å" /> '''RIBONUCLEASE INHIBITO... |
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[[Image:1dfj.jpg|left|200px]]<br /><applet load="1dfj" size=" | [[Image:1dfj.jpg|left|200px]]<br /><applet load="1dfj" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1dfj, resolution 2.5Å" /> | caption="1dfj, resolution 2.5Å" /> | ||
'''RIBONUCLEASE INHIBITOR COMPLEXED WITH RIBONUCLEASE A'''<br /> | '''RIBONUCLEASE INHIBITOR COMPLEXED WITH RIBONUCLEASE A'''<br /> | ||
==Overview== | ==Overview== | ||
The leucine-rich repeat is a recently characterized structural motif used | The leucine-rich repeat is a recently characterized structural motif used in molecular recognition processes as diverse as signal transduction, cell adhesion, cell development, DNA repair and RNA processing. We present here the crystal structure at 2.5 A resolution of the complex between ribonuclease A and ribonuclease inhibitor, a protein built entirely of leucine-rich repeats. The unusual non-globular structure of ribonuclease inhibitor, its solvent-exposed parallel beta-sheet and the conformational flexibility of the structure are used in the interaction; they appear to be the principal reasons for the effectiveness of leucine-rich repeats as protein-binding motifs. The structure can serve as a model for the interactions of other proteins containing leucine-rich repeats with their ligands. | ||
==About this Structure== | ==About this Structure== | ||
1DFJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with SO4 and ACE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http:// | 1DFJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=ACE:'>ACE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DFJ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: ribonuclease]] | [[Category: ribonuclease]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:16:10 2008'' | ||
Revision as of 13:16, 21 February 2008
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RIBONUCLEASE INHIBITOR COMPLEXED WITH RIBONUCLEASE A
OverviewOverview
The leucine-rich repeat is a recently characterized structural motif used in molecular recognition processes as diverse as signal transduction, cell adhesion, cell development, DNA repair and RNA processing. We present here the crystal structure at 2.5 A resolution of the complex between ribonuclease A and ribonuclease inhibitor, a protein built entirely of leucine-rich repeats. The unusual non-globular structure of ribonuclease inhibitor, its solvent-exposed parallel beta-sheet and the conformational flexibility of the structure are used in the interaction; they appear to be the principal reasons for the effectiveness of leucine-rich repeats as protein-binding motifs. The structure can serve as a model for the interactions of other proteins containing leucine-rich repeats with their ligands.
About this StructureAbout this Structure
1DFJ is a Protein complex structure of sequences from Bos taurus and Sus scrofa with and as ligands. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Full crystallographic information is available from OCA.
ReferenceReference
A structural basis of the interactions between leucine-rich repeats and protein ligands., Kobe B, Deisenhofer J, Nature. 1995 Mar 9;374(6518):183-6. PMID:7877692
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