1d8x: Difference between revisions
New page: left|200px<br /><applet load="1d8x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d8x, resolution 1.20Å" /> '''CRYSTAL STRUCTURE OF... |
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[[Image:1d8x.gif|left|200px]]<br /><applet load="1d8x" size=" | [[Image:1d8x.gif|left|200px]]<br /><applet load="1d8x" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1d8x, resolution 1.20Å" /> | caption="1d8x, resolution 1.20Å" /> | ||
'''CRYSTAL STRUCTURE OF DNA SHEARED TANDEM G A BASE PAIRS'''<br /> | '''CRYSTAL STRUCTURE OF DNA SHEARED TANDEM G A BASE PAIRS'''<br /> | ||
==Overview== | ==Overview== | ||
G x A mismatched base pairs are frequently found in nucleic acids. Human | G x A mismatched base pairs are frequently found in nucleic acids. Human centromere DNA sequences contain unusual repeating motifs, e.g. , (GAATG)n x (CATTC)n found in the human chromosome. The purine-rich strand of this repeating pentamer sequence forms duplex and hairpin structures with unusual stability. The high stability of these structures is contributed by the "sheared" G x A base pairs which present a novel recognition surface for ligands and proteins. We have solved the crystal structure, by the multiple-wavelength anomalous diffraction (MAD) method of d(CCGAATGAGG) in which the centromere core sequence motif GAATG is embedded. Three crystal forms were refined to near-atomic resolution. The structures reveal the detailed conformation of tandem G x A base pairs whose unique hydrogen-bonding surface has interesting interactions with bases, hydrated magnesium ions, cobalt(III)hexaammine, spermine, and water molecules. The results are relevant in understanding the structure associated with human centromere sequence in particular and G x A base pairs in nucleic acids (including RNA, like ribozyme) in general. | ||
==About this Structure== | ==About this Structure== | ||
1D8X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG and NCO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1D8X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=NCO:'>NCO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D8X OCA]. | ||
==Reference== | ==Reference== | ||
Structure and recognition of sheared tandem G x A base pairs associated with human centromere DNA sequence at atomic resolution., Gao YG, Robinson H, Sanishvili R, Joachimiak A, Wang AH, Biochemistry. 1999 Dec 14;38(50):16452-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10600106 10600106] | Structure and recognition of sheared tandem G x A base pairs associated with human centromere DNA sequence at atomic resolution., Gao YG, Robinson H, Sanishvili R, Joachimiak A, Wang AH, Biochemistry. 1999 Dec 14;38(50):16452-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10600106 10600106] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Gao, Y | [[Category: Gao, Y G.]] | ||
[[Category: Joachimiak, A.]] | [[Category: Joachimiak, A.]] | ||
[[Category: Robinson, H.]] | [[Category: Robinson, H.]] | ||
[[Category: Sanishvili, R.]] | [[Category: Sanishvili, R.]] | ||
[[Category: Wang, A | [[Category: Wang, A H.J.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
[[Category: NCO]] | [[Category: NCO]] | ||
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[[Category: tandem ga base pairs]] | [[Category: tandem ga base pairs]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:14:14 2008'' | ||
