1d92: Difference between revisions

Revision as of 13:14, 21 February 2008

REFINED CRYSTAL STRUCTURE OF AN OCTANUCLEOTIDE DUPLEX WITH G.T MISMATCHED BASE-PAIRS

OverviewOverview

Single crystal X-ray diffraction techniques have been used to determine the structure of the DNA octamer d(G-G-G-G-C-T-C-C) at a resolution of 2.25 A. The asymmetric unit consists of two strands coiled about each other to produce an A-type DNA helix. The double helix contains six G . C Watson-Crick base-pairs and two G . T mismatched base-pairs. The mismatches adopt a "wobble" type structure in which both bases retain their major tautomer forms. The double helix is able to accommodate this G . T pairing with little distortion of the overall helical conformation. Crystals of this octamer melt at a substantially lower temperature than do those of a related octamer also containing two G . T base-pairs. We attribute this destabilization to disruption of the hydration network around the mismatch site combined with changes in intermolecular packing. Full details are given of conformational parameters, base stacking, intermolecular contacts and hydration involving 52 solvent molecules.

About this StructureAbout this Structure

1D92 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

ReferenceReference

Refined crystal structure of an octanucleotide duplex with G . T mismatched base-pairs., Hunter WN, Kneale G, Brown T, Rabinovich D, Kennard O, J Mol Biol. 1986 Aug 20;190(4):605-18. PMID:3783714

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OCA

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-[[Image:1d92.gif|left|200px]]<br /><applet load="1d92" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1d92.gif|left|200px]]<br /><applet load="1d92" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1d92, resolution 2.250&Aring;" />
 '''REFINED CRYSTAL STRUCTURE OF AN OCTANUCLEOTIDE DUPLEX WITH G.T MISMATCHED BASE-PAIRS'''<br />
 ==Overview==
-Single crystal X-ray diffraction techniques have been used to determine, the structure of the DNA octamer d(G-G-G-G-C-T-C-C) at a resolution of, 2.25 A. The asymmetric unit consists of two strands coiled about each, other to produce an A-type DNA helix. The double helix contains six G . C, Watson-Crick base-pairs and two G . T mismatched base-pairs. The, mismatches adopt a "wobble" type structure in which both bases retain, their major tautomer forms. The double helix is able to accommodate this G, . T pairing with little distortion of the overall helical conformation., Crystals of this octamer melt at a substantially lower temperature than do, those of a related octamer also containing two G . T base-pairs. We, attribute this destabilization to disruption of the hydration network, around the mismatch site combined with changes in intermolecular packing., Full details are given of conformational parameters, base stacking, intermolecular contacts and hydration involving 52 solvent molecules.
+Single crystal X-ray diffraction techniques have been used to determine the structure of the DNA octamer d(G-G-G-G-C-T-C-C) at a resolution of 2.25 A. The asymmetric unit consists of two strands coiled about each other to produce an A-type DNA helix. The double helix contains six G . C Watson-Crick base-pairs and two G . T mismatched base-pairs. The mismatches adopt a "wobble" type structure in which both bases retain their major tautomer forms. The double helix is able to accommodate this G . T pairing with little distortion of the overall helical conformation. Crystals of this octamer melt at a substantially lower temperature than do those of a related octamer also containing two G . T base-pairs. We attribute this destabilization to disruption of the hydration network around the mismatch site combined with changes in intermolecular packing. Full details are given of conformational parameters, base stacking, intermolecular contacts and hydration involving 52 solvent molecules.
 ==About this Structure==
-D92 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D92 OCA].
+D92 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D92 OCA].
 ==Reference==
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 [[Category: Protein complex]]
 [[Category: Brown, T.]]
-[[Category: Hunter, W.N.]]
+[[Category: Hunter, W N.]]
 [[Category: Kennard, O.]]
 [[Category: Kneale, G.]]
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 [[Category: mismatched]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:47:17 2007''
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