1d8j: Difference between revisions

Revision as of 13:14, 21 February 2008

SOLUTION STRUCTURE OF THE CENTRAL CORE DOMAIN OF TFIIE BETA

OverviewOverview

Human general transcription factor TFIIE consists of two subunits, TFIIEalpha and TFIIEbeta. Recently, TFIIEbeta has been found to bind to the region where the promoter starts to open to be single-stranded upon transcription initiation by RNA polymerase II. Here, the central core domain of human TFIIEbeta (TFIIEbetac) has been identified by a limited proteolysis. This solution structure has been determined by NMR. It consists of three helices with a beta hairpin at the C-terminus, resembling the winged helix proteins. However, TFIIEbetac shows a novel double-stranded DNA-binding activity where the DNA-binding surface locates on the opposite side to the previously reported winged helix motif by forming a positively charged furrow. A model will be proposed that TFIIE stabilizes the preinitiation complex by binding not only to the general transcription factors together with RNA polymerase II but also to the promoter DNA, where double-stranded DNA starts to open to be single-stranded upon activation of the preinitiation complex.

About this StructureAbout this Structure

1D8J is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the central core domain of TFIIEbeta with a novel double-stranded DNA-binding surface., Okuda M, Watanabe Y, Okamura H, Hanaoka F, Ohkuma Y, Nishimura Y, EMBO J. 2000 Mar 15;19(6):1346-56. PMID:10716934

Page seeded by OCA on Thu Feb 21 12:14:10 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 '''SOLUTION STRUCTURE OF THE CENTRAL CORE DOMAIN OF TFIIE BETA'''<br />
 ==Overview==
-Human general transcription factor TFIIE consists of two subunits, TFIIEalpha and TFIIEbeta. Recently, TFIIEbeta has been found to bind to, the region where the promoter starts to open to be single-stranded upon, transcription initiation by RNA polymerase II. Here, the central core, domain of human TFIIEbeta (TFIIEbetac) has been identified by a limited, proteolysis. This solution structure has been determined by NMR. It, consists of three helices with a beta hairpin at the C-terminus, resembling the winged helix proteins. However, TFIIEbetac shows a novel, double-stranded DNA-binding activity where the DNA-binding surface locates, on the opposite side to the previously reported winged helix motif by, forming a positively charged furrow. A model will be proposed that TFIIE, stabilizes the preinitiation complex by binding not only to the general, transcription factors together with RNA polymerase II but also to the, promoter DNA, where double-stranded DNA starts to open to be, single-stranded upon activation of the preinitiation complex.
+Human general transcription factor TFIIE consists of two subunits, TFIIEalpha and TFIIEbeta. Recently, TFIIEbeta has been found to bind to the region where the promoter starts to open to be single-stranded upon transcription initiation by RNA polymerase II. Here, the central core domain of human TFIIEbeta (TFIIEbetac) has been identified by a limited proteolysis. This solution structure has been determined by NMR. It consists of three helices with a beta hairpin at the C-terminus, resembling the winged helix proteins. However, TFIIEbetac shows a novel double-stranded DNA-binding activity where the DNA-binding surface locates on the opposite side to the previously reported winged helix motif by forming a positively charged furrow. A model will be proposed that TFIIE stabilizes the preinitiation complex by binding not only to the general transcription factors together with RNA polymerase II but also to the promoter DNA, where double-stranded DNA starts to open to be single-stranded upon activation of the preinitiation complex.
 ==About this Structure==
-D8J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D8J OCA].
+D8J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D8J OCA].
 ==Reference==
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 [[Category: Okamura, H.]]
 [[Category: Okuda, M.]]
-[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
+[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
 [[Category: Watanabe, Y.]]
 [[Category: riken structural genomics/proteomics initiative]]
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 [[Category: winged helix-turn-helix]]
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