1d7p: Difference between revisions
New page: left|200px<br /> <applet load="1d7p" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d7p, resolution 1.5Å" /> '''Crystal structure of... |
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[[Image:1d7p.gif|left|200px]]<br /> | [[Image:1d7p.gif|left|200px]]<br /><applet load="1d7p" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="1d7p, resolution 1.5Å" /> | caption="1d7p, resolution 1.5Å" /> | ||
'''Crystal structure of the c2 domain of human factor viii at 1.5 a resolution at 1.5 A'''<br /> | '''Crystal structure of the c2 domain of human factor viii at 1.5 a resolution at 1.5 A'''<br /> | ||
==Overview== | ==Overview== | ||
Human factor VIII is a plasma glycoprotein that has a critical role in | Human factor VIII is a plasma glycoprotein that has a critical role in blood coagulation. Factor VIII circulates as a complex with von Willebrand factor. After cleavage by thrombin, factor VIIIa associates with factor IXa at the surface of activated platelets or endothelial cells. This complex activates factor X (refs 6, 7), which in turn converts prothrombin to thrombin in the presence of factor Va (refs 8, 9). The carboxyl-terminal C2 domain of factor VIII contains sites that are essential for its binding to von Willebrand factor and to negatively charged phospholipid surfaces. Here we report the structure of human factor VIII C2 domain at 1.5 A resolution. The structure reveals a beta-sandwich core, from which two beta-turns and a loop display a group of solvent-exposed hydrophobic residues. Behind the hydrophobic surface lies a ring of positively charged residues. This motif suggests a mechanism for membrane binding involving both hydrophobic and electrostatic interactions. The structure explains, in part, mutations in the C2 region of factor VIII that lead to bleeding disorders in haemophilia A. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
1D7P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4, CYS and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1D7P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=CYS:'>CYS</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D7P OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Pratt, K | [[Category: Pratt, K P.]] | ||
[[Category: Shen, B | [[Category: Shen, B W.]] | ||
[[Category: Stoddard, B | [[Category: Stoddard, B L.]] | ||
[[Category: CYS]] | [[Category: CYS]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
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[[Category: blood clotting]] | [[Category: blood clotting]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:13:50 2008'' |