1b4w: Difference between revisions
New page: left|200px<br /> <applet load="1b4w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b4w, resolution 2.60Å" /> '''BASIC PHOSPHOLIPASE... |
No edit summary |
||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
1B4W is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Gloydius_halys Gloydius halys]] with BOG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1B4W OCA]]. | 1B4W is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Gloydius_halys Gloydius halys]] with BOG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4]]. Structure known Active Sites: AC1, AC2, AC3 and AC4. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1B4W OCA]]. | ||
==Reference== | ==Reference== | ||
Structure of basic phospholipase A2 from Agkistrodon halys Pallas: implications for its association, hemolytic and anticoagulant activities., Zhao K, Zhou Y, Lin Z, Toxicon. 2000 Jul;38(7):901-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10728829 10728829] | Structure of basic phospholipase A2 from Agkistrodon halys Pallas: implications for its association, hemolytic and anticoagulant activities., Zhao K, Zhou Y, Lin Z, Toxicon. 2000 Jul;38(7):901-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10728829 10728829] | ||
[[Category: Gloydius halys]] | [[Category: Gloydius halys]] | ||
[[Category: Phospholipase A(2)]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Lin, Z.J.]] | [[Category: Lin, Z.J.]] | ||
| Line 22: | Line 23: | ||
[[Category: dimer]] | [[Category: dimer]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:49:02 2007'' | ||
Revision as of 14:44, 30 October 2007
|
BASIC PHOSPHOLIPASE A2 FROM AGKISTRODON HALYS PALLAS-IMPLICATIONS FOR ITS ASSOCIATION AND ANTICOAGULANT ACTIVITIES BY X-RAY CRYSTALLOGRAPHY
OverviewOverview
The basic phospholipase A2 (PLA2) from the venom of Agkistrodon halys, Pallas is a potent hemolytic toxin and anticoagulant. Crystal structure of, the enzyme complexed with detergent n-octyl beta-D-glucopyranoside, (beta-OG) in monoclinic crystal form has been determined to 2.6 A, resolution. Beta-OG molecules were found in the hydrophobic channels of, the enzyme. SDS-PAGE and dynamic light scattering measurements showed that, the enzyme had a strong tendency to dimerise in aqueous solution. In the, crystal structure the enzyme molecules associate into a tetramer with, pseudo 222 symmetry, and the interfacial recognition site linked dimers, constituting the tetramer have intensive interface interactions, and may, be stable in solution. The structure reveals a unique positively charged, ... [(full description)]
About this StructureAbout this Structure
1B4W is a [Single protein] structure of sequence from [Gloydius halys] with BOG as [ligand]. Active as [Phospholipase A(2)], with EC number [3.1.1.4]. Structure known Active Sites: AC1, AC2, AC3 and AC4. Full crystallographic information is available from [OCA].
ReferenceReference
Structure of basic phospholipase A2 from Agkistrodon halys Pallas: implications for its association, hemolytic and anticoagulant activities., Zhao K, Zhou Y, Lin Z, Toxicon. 2000 Jul;38(7):901-16. PMID:10728829
Page seeded by OCA on Tue Oct 30 13:49:02 2007