1d4c: Difference between revisions

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CRYSTAL STRUCTURE OF THE UNCOMPLEXED FORM OF THE FLAVOCYTOCHROME C FUMARATE REDUCTASE OF SHEWANELLA PUTREFACIENS STRAIN MR-1

OverviewOverview

Fumarate respiration is one of the most widespread types of anaerobic respiration. The soluble fumarate reductase of Shewanella putrefaciens MR-1 is a periplasmic tetraheme flavocytochrome c. The crystal structures of the enzyme were solved to 2.9 A for the uncomplexed form and to 2.8 A and 2.5 A for the fumarate and the succinate-bound protein, respectively. The structures reveal a flexible capping domain linked to the FAD-binding domain. A catalytic mechanism for fumarate reduction based on the structure of the complexed protein is proposed. The mechanism for the reverse reaction is a model for the homologous succinate dehydrogenase (complex II) of the respiratory chain. In flavocytochrome c fumarate reductase, all redox centers are in van der Waals contact with one another, thus providing an efficient conduit of electrons from the hemes via the FAD to fumarate.

About this StructureAbout this Structure

1D4C is a Single protein structure of sequence from Shewanella putrefaciens with , and as ligands. Active as Succinate dehydrogenase, with EC number 1.3.99.1 Full crystallographic information is available from OCA.

ReferenceReference

Structure and mechanism of the flavocytochrome c fumarate reductase of Shewanella putrefaciens MR-1., Leys D, Tsapin AS, Nealson KH, Meyer TE, Cusanovich MA, Van Beeumen JJ, Nat Struct Biol. 1999 Dec;6(12):1113-7. PMID:10581551

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-[[Image:1d4c.jpg|left|200px]]<br /><applet load="1d4c" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1d4c.jpg|left|200px]]<br /><applet load="1d4c" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1d4c, resolution 2.9&Aring;" />
 '''CRYSTAL STRUCTURE OF THE UNCOMPLEXED FORM OF THE FLAVOCYTOCHROME C FUMARATE REDUCTASE OF SHEWANELLA PUTREFACIENS STRAIN MR-1'''<br />
 ==Overview==
-Fumarate respiration is one of the most widespread types of anaerobic, respiration. The soluble fumarate reductase of Shewanella putrefaciens, MR-1 is a periplasmic tetraheme flavocytochrome c. The crystal structures, of the enzyme were solved to 2.9 A for the uncomplexed form and to 2.8 A, and 2.5 A for the fumarate and the succinate-bound protein, respectively., The structures reveal a flexible capping domain linked to the FAD-binding, domain. A catalytic mechanism for fumarate reduction based on the, structure of the complexed protein is proposed. The mechanism for the, reverse reaction is a model for the homologous succinate dehydrogenase, (complex II) of the respiratory chain. In flavocytochrome c fumarate, reductase, all redox centers are in van der Waals contact with one, another, thus providing an efficient conduit of electrons from the hemes, via the FAD to fumarate.
+Fumarate respiration is one of the most widespread types of anaerobic respiration. The soluble fumarate reductase of Shewanella putrefaciens MR-1 is a periplasmic tetraheme flavocytochrome c. The crystal structures of the enzyme were solved to 2.9 A for the uncomplexed form and to 2.8 A and 2.5 A for the fumarate and the succinate-bound protein, respectively. The structures reveal a flexible capping domain linked to the FAD-binding domain. A catalytic mechanism for fumarate reduction based on the structure of the complexed protein is proposed. The mechanism for the reverse reaction is a model for the homologous succinate dehydrogenase (complex II) of the respiratory chain. In flavocytochrome c fumarate reductase, all redox centers are in van der Waals contact with one another, thus providing an efficient conduit of electrons from the hemes via the FAD to fumarate.
 ==About this Structure==
-D4C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Shewanella_putrefaciens Shewanella putrefaciens] with SO4, HEM and FAD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Succinate_dehydrogenase Succinate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.99.1 1.3.99.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D4C OCA].
+D4C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Shewanella_putrefaciens Shewanella putrefaciens] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=FAD:'>FAD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Succinate_dehydrogenase Succinate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.99.1 1.3.99.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D4C OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Succinate dehydrogenase]]
-[[Category: Beeumen, J.J.Van.]]
+[[Category: Beeumen, J J.Van.]]
-[[Category: Cusanovich, M.A.]]
+[[Category: Cusanovich, M A.]]
 [[Category: Leys, D.]]
-[[Category: Meyer, T.E.]]
+[[Category: Meyer, T E.]]
-[[Category: Tsapin, A.S.]]
+[[Category: Tsapin, A S.]]
 [[Category: FAD]]
 [[Category: HEM]]
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 [[Category: tetraheme flavocytochrome c]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:59:55 2007''
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