1d3y: Difference between revisions

Revision as of 13:12, 21 February 2008

STRUCTURE OF THE DNA TOPOISOMERASE VI A SUBUNIT

OverviewOverview

In all organisms, type II DNA topoisomerases are essential for untangling chromosomal DNA. We have determined the structure of the DNA-binding core of the Methanococcus jannaschii DNA topoisomerase VI A subunit at 2.0 A resolution. The overall structure of this subunit is unique, demonstrating that archaeal type II enzymes are distinct from other type II topoisomerases. However, the core structure contains a pair of domains that are also found in type IA and classic type II topoisomerases. Together, these regions may form the basis of a DNA cleavage mechanism shared among these enzymes. The core A subunit is a dimer that contains a deep groove that spans both protomers. The dimer architecture suggests that DNA is bound in the groove, across the A subunit interface, and that the two monomers separate during DNA transport. The A subunit of topoisomerase VI is homologous to the meiotic recombination factor, Spo11, and this structure can serve as a template for probing Spo11 function in eukaryotes.

About this StructureAbout this Structure

1D3Y is a Single protein structure of sequence from Methanocaldococcus jannaschii with and as ligands. Active as DNA topoisomerase (ATP-hydrolyzing), with EC number 5.99.1.3 Full crystallographic information is available from OCA.

ReferenceReference

Structure and function of an archaeal topoisomerase VI subunit with homology to the meiotic recombination factor Spo11., Nichols MD, DeAngelis K, Keck JL, Berger JM, EMBO J. 1999 Nov 1;18(21):6177-88. PMID:10545127

Page seeded by OCA on Thu Feb 21 12:12:45 2008

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OCA

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-[[Image:1d3y.gif|left|200px]]<br /><applet load="1d3y" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1d3y.gif|left|200px]]<br /><applet load="1d3y" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1d3y, resolution 2.0&Aring;" />
 '''STRUCTURE OF THE DNA TOPOISOMERASE VI A SUBUNIT'''<br />
 ==Overview==
-In all organisms, type II DNA topoisomerases are essential for untangling, chromosomal DNA. We have determined the structure of the DNA-binding core, of the Methanococcus jannaschii DNA topoisomerase VI A subunit at 2.0 A, resolution. The overall structure of this subunit is unique, demonstrating, that archaeal type II enzymes are distinct from other type II, topoisomerases. However, the core structure contains a pair of domains, that are also found in type IA and classic type II topoisomerases., Together, these regions may form the basis of a DNA cleavage mechanism, shared among these enzymes. The core A subunit is a dimer that contains a, deep groove that spans both protomers. The dimer architecture suggests, that DNA is bound in the groove, across the A subunit interface, and that, the two monomers separate during DNA transport. The A subunit of, topoisomerase VI is homologous to the meiotic recombination factor, Spo11, and this structure can serve as a template for probing Spo11 function in, eukaryotes.
+In all organisms, type II DNA topoisomerases are essential for untangling chromosomal DNA. We have determined the structure of the DNA-binding core of the Methanococcus jannaschii DNA topoisomerase VI A subunit at 2.0 A resolution. The overall structure of this subunit is unique, demonstrating that archaeal type II enzymes are distinct from other type II topoisomerases. However, the core structure contains a pair of domains that are also found in type IA and classic type II topoisomerases. Together, these regions may form the basis of a DNA cleavage mechanism shared among these enzymes. The core A subunit is a dimer that contains a deep groove that spans both protomers. The dimer architecture suggests that DNA is bound in the groove, across the A subunit interface, and that the two monomers separate during DNA transport. The A subunit of topoisomerase VI is homologous to the meiotic recombination factor, Spo11, and this structure can serve as a template for probing Spo11 function in eukaryotes.
 ==About this Structure==
-D3Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with NA and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA_topoisomerase_(ATP-hydrolyzing) DNA topoisomerase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.3 5.99.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D3Y OCA].
+D3Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA_topoisomerase_(ATP-hydrolyzing) DNA topoisomerase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.3 5.99.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D3Y OCA].
 ==Reference==
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 [[Category: Methanocaldococcus jannaschii]]
 [[Category: Single protein]]
-[[Category: Berger, J.M.]]
+[[Category: Berger, J M.]]
-[[Category: DeAngelis, K.A.]]
+[[Category: DeAngelis, K A.]]
-[[Category: Keck, J.L.]]
+[[Category: Keck, J L.]]
-[[Category: Nichols, M.D.]]
+[[Category: Nichols, M D.]]
 [[Category: MG]]
 [[Category: NA]]
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 [[Category: topoisomerase vi]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:59:48 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:12:45 2008''