1d29: Difference between revisions

Revision as of 13:12, 21 February 2008

CRYSTAL STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACG

OverviewOverview

The crystal structure of the DNA dodecamer C-G-T-G-A-A-T-T-C-A-C-G has been determined at a resolution of 2.5 A, with a final R factor of 15.8% for 1475 nonzero reflections measured at 0 degrees C. The structure is isomorphous with that of the Drew dodecamer, with the space group P2(1)2(1)2(1) and cell dimensions of a = 24.94 A, b = 40.78 A, and c = 66.13 A. The asymmetric unit contains all 12 base pairs of the B-DNA double helix and 36 water molecules. The structure of C-G-T-G-A-A-T-T-C-A-C-G is very similar to that of C-G-C-G-A-A-T-T-C-G-C-G, with no major alterations in helix parameters. Water peaks in the refined structure appear to represent a selection of peaks that were observed in the Drew dodecamer. The minor-groove spine of hydration at 2.5 A is fragmentary, but as Narendra et al. (1991) [Biochemistry (following paper in this issue)] have observed, lowering the temperature leads to a more complete representation of the spine.

About this StructureAbout this Structure

1D29 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure analysis of the B-DNA dodecamer CGTGAATTCACG., Larsen TA, Kopka ML, Dickerson RE, Biochemistry. 1991 May 7;30(18):4443-9. PMID:1850624

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OCA

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-[[Image:1d29.gif|left|200px]]<br /><applet load="1d29" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1d29.gif|left|200px]]<br /><applet load="1d29" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1d29, resolution 2.500&Aring;" />
 '''CRYSTAL STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACG'''<br />
 ==Overview==
-The crystal structure of the DNA dodecamer C-G-T-G-A-A-T-T-C-A-C-G has, been determined at a resolution of 2.5 A, with a final R factor of 15.8%, for 1475 nonzero reflections measured at 0 degrees C. The structure is, isomorphous with that of the Drew dodecamer, with the space group, P2(1)2(1)2(1) and cell dimensions of a = 24.94 A, b = 40.78 A, and c =, 66.13 A. The asymmetric unit contains all 12 base pairs of the B-DNA, double helix and 36 water molecules. The structure of, C-G-T-G-A-A-T-T-C-A-C-G is very similar to that of, C-G-C-G-A-A-T-T-C-G-C-G, with no major alterations in helix parameters., Water peaks in the refined structure appear to represent a selection of, peaks that were observed in the Drew dodecamer. The minor-groove spine of, hydration at 2.5 A is fragmentary, but as Narendra et al. (1991), [Biochemistry (following paper in this issue)] have observed, lowering the, temperature leads to a more complete representation of the spine.
+The crystal structure of the DNA dodecamer C-G-T-G-A-A-T-T-C-A-C-G has been determined at a resolution of 2.5 A, with a final R factor of 15.8% for 1475 nonzero reflections measured at 0 degrees C. The structure is isomorphous with that of the Drew dodecamer, with the space group P2(1)2(1)2(1) and cell dimensions of a = 24.94 A, b = 40.78 A, and c = 66.13 A. The asymmetric unit contains all 12 base pairs of the B-DNA double helix and 36 water molecules. The structure of C-G-T-G-A-A-T-T-C-A-C-G is very similar to that of C-G-C-G-A-A-T-T-C-G-C-G, with no major alterations in helix parameters. Water peaks in the refined structure appear to represent a selection of peaks that were observed in the Drew dodecamer. The minor-groove spine of hydration at 2.5 A is fragmentary, but as Narendra et al. (1991) [Biochemistry (following paper in this issue)] have observed, lowering the temperature leads to a more complete representation of the spine.
 ==About this Structure==
-D29 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D29 OCA].
+D29 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D29 OCA].
 ==Reference==
 Crystal structure analysis of the B-DNA dodecamer CGTGAATTCACG., Larsen TA, Kopka ML, Dickerson RE, Biochemistry. 1991 May 7;30(18):4443-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1850624 1850624]
 [[Category: Protein complex]]
-[[Category: Dickerson, R.E.]]
+[[Category: Dickerson, R E.]]
-[[Category: Kopka, M.L.]]
+[[Category: Kopka, M L.]]
-[[Category: Larsen, T.A.]]
+[[Category: Larsen, T A.]]
 [[Category: b-dna]]
 [[Category: double helix]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:19:36 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:12:10 2008''