1cw0: Difference between revisions

Revision as of 13:10, 21 February 2008

CRYSTAL STRUCTURE ANALYSIS OF VERY SHORT PATCH REPAIR (VSR) ENDONUCLEASE IN COMPLEX WITH A DUPLEX DNA

OverviewOverview

The crystal structure of very short patch repair (Vsr) endonuclease, in complex with Mg2+ and with duplex DNA containing a TG mismatch, has been determined at 2.3 A resolution. In E. coli, the enzyme recognizes a TG mismatched base pair, generated after spontaneous deamination of methylated cytosines, and cleaves the phosphate backbone on the 5' side of the thymine. Extensive interactions between the DNA and the protein characterize a novel recognition mechanism, where three aromatic residues intercalate from the major groove into the DNA to strikingly deform the base pair stacking. With the presence of a cleaved DNA intermediate in the active center, the structure of the Vsr/DNA complex provides detailed insights into the catalytic mechanism for endonuclease activity.

About this StructureAbout this Structure

1CW0 is a Single protein structure of sequence from Escherichia coli with and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Recognition of a TG mismatch: the crystal structure of very short patch repair endonuclease in complex with a DNA duplex., Tsutakawa SE, Jingami H, Morikawa K, Cell. 1999 Dec 10;99(6):615-23. PMID:10612397

Page seeded by OCA on Thu Feb 21 12:10:19 2008

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OCA

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-[[Image:1cw0.gif|left|200px]]<br /><applet load="1cw0" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1cw0.gif|left|200px]]<br /><applet load="1cw0" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1cw0, resolution 2.30&Aring;" />
 '''CRYSTAL STRUCTURE ANALYSIS OF VERY SHORT PATCH REPAIR (VSR) ENDONUCLEASE IN COMPLEX WITH A DUPLEX DNA'''<br />
 ==Overview==
-The crystal structure of very short patch repair (Vsr) endonuclease, in, complex with Mg2+ and with duplex DNA containing a TG mismatch, has been, determined at 2.3 A resolution. In E. coli, the enzyme recognizes a TG, mismatched base pair, generated after spontaneous deamination of, methylated cytosines, and cleaves the phosphate backbone on the 5' side of, the thymine. Extensive interactions between the DNA and the protein, characterize a novel recognition mechanism, where three aromatic residues, intercalate from the major groove into the DNA to strikingly deform the, base pair stacking. With the presence of a cleaved DNA intermediate in the, active center, the structure of the Vsr/DNA complex provides detailed, insights into the catalytic mechanism for endonuclease activity.
+The crystal structure of very short patch repair (Vsr) endonuclease, in complex with Mg2+ and with duplex DNA containing a TG mismatch, has been determined at 2.3 A resolution. In E. coli, the enzyme recognizes a TG mismatched base pair, generated after spontaneous deamination of methylated cytosines, and cleaves the phosphate backbone on the 5' side of the thymine. Extensive interactions between the DNA and the protein characterize a novel recognition mechanism, where three aromatic residues intercalate from the major groove into the DNA to strikingly deform the base pair stacking. With the presence of a cleaved DNA intermediate in the active center, the structure of the Vsr/DNA complex provides detailed insights into the catalytic mechanism for endonuclease activity.
 ==About this Structure==
-CW0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CW0 OCA].
+CW0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CW0 OCA].
 ==Reference==
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 [[Category: Jingami, H.]]
 [[Category: Morikawa, K.]]
-[[Category: Tsutakawa, S.E.]]
+[[Category: Tsutakawa, S E.]]
 [[Category: MG]]
 [[Category: ZN]]
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 [[Category: zinc]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:47:44 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:10:19 2008''