1csg: Difference between revisions
New page: left|200px<br /> <applet load="1csg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1csg, resolution 2.7Å" /> '''THREE-DIMENSIONAL ST... |
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[[Image:1csg.gif|left|200px]]<br /> | [[Image:1csg.gif|left|200px]]<br /><applet load="1csg" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="1csg, resolution 2.7Å" /> | caption="1csg, resolution 2.7Å" /> | ||
'''THREE-DIMENSIONAL STRUCTURE OF RECOMBINANT HUMAN GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR'''<br /> | '''THREE-DIMENSIONAL STRUCTURE OF RECOMBINANT HUMAN GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR'''<br /> | ||
==Overview== | ==Overview== | ||
The crystal structure of recombinant human granulocyte-macrophage | The crystal structure of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) has been determined at 2.8 A resolution using multiple isomorphous replacement techniques. There are two molecules in the crystallographic asymmetric unit, which are related by an approximate non-crystallographic 2-fold axis. The overall structure is highly compact and globular with a predominantly hydrophobic core. The main structural feature of rhGM-CSF is a four alpha-helix bundle, which represents approximately 42% of the structure. The helices are arranged in a left-handed antiparallel bundle with two overhand connections. Within the connections is a two-stranded antiparallel beta-sheet. The tertiary structure of rhGM-CSF has a topology similar to that of porcine growth factor and interferon-beta. Most of the proposed critical regions for receptor binding are located on a continuous surface at one end of the molecule that includes the C terminus. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
1CSG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | 1CSG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CSG OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Cook, W | [[Category: Cook, W J.]] | ||
[[Category: Ealick, S | [[Category: Ealick, S E.]] | ||
[[Category: Walter, M | [[Category: Walter, M R.]] | ||
[[Category: cytokine]] | [[Category: cytokine]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:09:17 2008'' | ||
Revision as of 13:09, 21 February 2008
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THREE-DIMENSIONAL STRUCTURE OF RECOMBINANT HUMAN GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR
OverviewOverview
The crystal structure of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) has been determined at 2.8 A resolution using multiple isomorphous replacement techniques. There are two molecules in the crystallographic asymmetric unit, which are related by an approximate non-crystallographic 2-fold axis. The overall structure is highly compact and globular with a predominantly hydrophobic core. The main structural feature of rhGM-CSF is a four alpha-helix bundle, which represents approximately 42% of the structure. The helices are arranged in a left-handed antiparallel bundle with two overhand connections. Within the connections is a two-stranded antiparallel beta-sheet. The tertiary structure of rhGM-CSF has a topology similar to that of porcine growth factor and interferon-beta. Most of the proposed critical regions for receptor binding are located on a continuous surface at one end of the molecule that includes the C terminus.
DiseaseDisease
Known disease associated with this structure: Leukemia, acute myeloid, M2 type OMIM:[306250]
About this StructureAbout this Structure
1CSG is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Three-dimensional structure of recombinant human granulocyte-macrophage colony-stimulating factor., Walter MR, Cook WJ, Ealick SE, Nagabhushan TL, Trotta PP, Bugg CE, J Mol Biol. 1992 Apr 20;224(4):1075-85. PMID:1569568
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