1crk: Difference between revisions

Revision as of 13:09, 21 February 2008

MITOCHONDRIAL CREATINE KINASE

OverviewOverview

Creatine kinase (CK, EC 2.7.3.2), an enzyme important for energy metabolism in cells of high and fluctuating energy requirements, catalyses the reversible transfer of a phosphoryl goup from phosphocreatine to ADP. We have solved the structure of the octameric mitochondrial isoform, Mib-CK, which is located in the intermembrane compartment and along the cristae membranes. Mib-CK consumes ATP produced in the mitochondria for the production of phosphocreatine, which is then exported into the cytosol for fast regeneration of ATP by the cytosolic CK isoforms. The octamer has 422 point-group symmetry, and appears as a cube of side length 93 angstrom with a channel 20 angstrom wide extending along the four-fold axis. Positively charged amino acids at the four-fold faces of the octamer possibly interact with negatively charged mitochondrial membranes. Each monomer consists of a small alpha-helical domain and a large domain containing an eight-stranded antiparallel beta-sheet flanked by seven alpha-helices. The conserved residues of the CK family form a compact cluster that covers the active site between the domains.

About this StructureAbout this Structure

1CRK is a Single protein structure of sequence from Gallus gallus with as ligand. Active as Creatine kinase, with EC number 2.7.3.2 Full crystallographic information is available from OCA.

ReferenceReference

Structure of mitochondrial creatine kinase., Fritz-Wolf K, Schnyder T, Wallimann T, Kabsch W, Nature. 1996 May 23;381(6580):341-5. PMID:8692275

Page seeded by OCA on Thu Feb 21 12:09:01 2008

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OCA

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 '''MITOCHONDRIAL CREATINE KINASE'''<br />
 ==Overview==
-Creatine kinase (CK, EC 2.7.3.2), an enzyme important for energy, metabolism in cells of high and fluctuating energy requirements, catalyses, the reversible transfer of a phosphoryl goup from phosphocreatine to ADP., We have solved the structure of the octameric mitochondrial isoform, Mib-CK, which is located in the intermembrane compartment and along the, cristae membranes. Mib-CK consumes ATP produced in the mitochondria for, the production of phosphocreatine, which is then exported into the cytosol, for fast regeneration of ATP by the cytosolic CK isoforms. The octamer has, 422 point-group symmetry, and appears as a cube of side length 93 angstrom, with a channel 20 angstrom wide extending along the four-fold axis., Positively charged amino acids at the four-fold faces of the octamer, possibly interact with negatively charged mitochondrial membranes. Each, monomer consists of a small alpha-helical domain and a large domain, containing an eight-stranded antiparallel beta-sheet flanked by seven, alpha-helices. The conserved residues of the CK family form a compact, cluster that covers the active site between the domains.
+Creatine kinase (CK, EC 2.7.3.2), an enzyme important for energy metabolism in cells of high and fluctuating energy requirements, catalyses the reversible transfer of a phosphoryl goup from phosphocreatine to ADP. We have solved the structure of the octameric mitochondrial isoform, Mib-CK, which is located in the intermembrane compartment and along the cristae membranes. Mib-CK consumes ATP produced in the mitochondria for the production of phosphocreatine, which is then exported into the cytosol for fast regeneration of ATP by the cytosolic CK isoforms. The octamer has 422 point-group symmetry, and appears as a cube of side length 93 angstrom with a channel 20 angstrom wide extending along the four-fold axis. Positively charged amino acids at the four-fold faces of the octamer possibly interact with negatively charged mitochondrial membranes. Each monomer consists of a small alpha-helical domain and a large domain containing an eight-stranded antiparallel beta-sheet flanked by seven alpha-helices. The conserved residues of the CK family form a compact cluster that covers the active site between the domains.
 ==About this Structure==
-CRK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Creatine_kinase Creatine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.3.2 2.7.3.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CRK OCA].
+CRK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Creatine_kinase Creatine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.3.2 2.7.3.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRK OCA].
 ==Reference==
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 [[Category: transferase]]
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