1cpc: Difference between revisions

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-[[Image:1cpc.gif|left|200px]]<br /><applet load="1cpc" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1cpc.gif|left|200px]]<br /><applet load="1cpc" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1cpc, resolution 1.66&Aring;" />
 '''ISOLATION, CRYSTALLIZATION, CRYSTAL STRUCTURE ANALYSIS AND REFINEMENT OF CONSTITUTIVE C-PHYCOCYANIN FROM THE CHROMATICALLY ADAPTING CYANOBACTERIUM FREMYELLA DIPLOSIPHON AT 1.66 ANGSTROMS RESOLUTION'''<br />
 ==Overview==
-Constitutive phycocyanin from cyanobacterium Fremyella diplosiphon, (Calothrix sp. PCC 7601) grown in green light, has been isolated and, crystallized. The crystals belong to the space group R3 with cell, constants a = b = 180.26 A, c = 61.24 A, alpha = beta = 90 degrees, gamma, = 120 degrees. The crystal structure has been determined by Patterson, search techniques using the molecular model of C-phycocyanin from the, cyanobacterium Agmenellum quadruplicatum. The asymmetric unit of the, crystal cell consists of two (alpha beta)-monomers related by a local, dyad. Three asymmetric units are arranged around a crystallographic triad, and form an (alpha beta)6-hexamer, the functional unit in the native, antenna rod. The initial structure has been refined in a cyclic manner by, energy-restrained crystallographic refinement and modelling until the, conventional crystallographic R-factor converged at 18.1% with data to a, resolution of 1.66 A. The molecular structure resembles closely the, C-phycocyanins of Mastigocladus laminosus and A. quadruplicatum. The, conformation and configuration of the alpha-84 and beta-84 chromophores is, very similar to the corresponding chromophores in the trimeric, C-phycocyanin of M. laminosus, whereas the beta-155 chromophore differs in, configuration with C(4)-Z, C(10)-Z and C(15)-Z compared to C(4)-Z, C(10)-Z, C(15)-Z,E. The stereochemistry of the beta-155 chiral centres is, C(2)-RC(3)-R and C(31)-S, respectively, whereas alpha-84 and beta-84 have, C(2)-RC(3)-R and C(31)-R. The amino acid sequences of constitutive and, inducible phycocyanin differ mainly in residues located on the surface of, the beta-subunits that mediate the inter-hexameric contacts.
+Constitutive phycocyanin from cyanobacterium Fremyella diplosiphon (Calothrix sp. PCC 7601) grown in green light, has been isolated and crystallized. The crystals belong to the space group R3 with cell constants a = b = 180.26 A, c = 61.24 A, alpha = beta = 90 degrees, gamma = 120 degrees. The crystal structure has been determined by Patterson search techniques using the molecular model of C-phycocyanin from the cyanobacterium Agmenellum quadruplicatum. The asymmetric unit of the crystal cell consists of two (alpha beta)-monomers related by a local dyad. Three asymmetric units are arranged around a crystallographic triad and form an (alpha beta)6-hexamer, the functional unit in the native antenna rod. The initial structure has been refined in a cyclic manner by energy-restrained crystallographic refinement and modelling until the conventional crystallographic R-factor converged at 18.1% with data to a resolution of 1.66 A. The molecular structure resembles closely the C-phycocyanins of Mastigocladus laminosus and A. quadruplicatum. The conformation and configuration of the alpha-84 and beta-84 chromophores is very similar to the corresponding chromophores in the trimeric C-phycocyanin of M. laminosus, whereas the beta-155 chromophore differs in configuration with C(4)-Z, C(10)-Z and C(15)-Z compared to C(4)-Z, C(10)-Z, C(15)-Z,E. The stereochemistry of the beta-155 chiral centres is C(2)-RC(3)-R and C(31)-S, respectively, whereas alpha-84 and beta-84 have C(2)-RC(3)-R and C(31)-R. The amino acid sequences of constitutive and inducible phycocyanin differ mainly in residues located on the surface of the beta-subunits that mediate the inter-hexameric contacts.
 ==About this Structure==
-CPC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Fremyella_diplosiphon Fremyella diplosiphon] with CYC and CH2 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CPC OCA].
+CPC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Fremyella_diplosiphon Fremyella diplosiphon] with <scene name='pdbligand=CYC:'>CYC</scene> and <scene name='pdbligand=CH2:'>CH2</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CPC OCA].
 ==Reference==
@@ Line 15: / Line 15: @@
 [[Category: Duerring, M.]]
 [[Category: Huber, R.]]
-[[Category: Schmidt, G.B.]]
+[[Category: Schmidt, G B.]]
 [[Category: CH2]]
 [[Category: CYC]]
 [[Category: light harvesting protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:38:42 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:08:20 2008''