1cft: Difference between revisions

Revision as of 13:05, 21 February 2008

ANTI-P24 (HIV-1) FAB FRAGMENT CB41 COMPLEXED WITH AN EPITOPE-UNRELATED D-PEPTIDE

OverviewOverview

The X-ray crystal structures of an anti-p24 (HIV-1) monoclonal antibody Fab fragment alone and in complexes with the epitope peptide GATPQDLNTnL (n = norleucine), an epitope-homologous peptide GATPEDLNQKLAGN, as well as two unrelated peptides GLYEWGGARITNTD and efslkGpllqwrsG (D-peptide), are presented to a maximum resolution of 2.6 A. The latter three peptides were identified from screening synthetic combinatorial peptide libraries. Although all peptides bind to the same antigen combining site, the nonhomologous peptides adopt different binding conformations and also form their critical contacts with different antibody residues. Only small readjustments are observed within the framework of the Fab fragment upon binding.

About this StructureAbout this Structure

1CFT is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Crystallographic analysis of anti-p24 (HIV-1) monoclonal antibody cross-reactivity and polyspecificity., Keitel T, Kramer A, Wessner H, Scholz C, Schneider-Mergener J, Hohne W, Cell. 1997 Dec 12;91(6):811-20. PMID:9413990

Page seeded by OCA on Thu Feb 21 12:05:41 2008

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OCA

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 ==Overview==
-The X-ray crystal structures of an anti-p24 (HIV-1) monoclonal antibody, Fab fragment alone and in complexes with the epitope peptide GATPQDLNTnL, (n = norleucine), an epitope-homologous peptide GATPEDLNQKLAGN, as well as, two unrelated peptides GLYEWGGARITNTD and efslkGpllqwrsG (D-peptide), are, presented to a maximum resolution of 2.6 A. The latter three peptides were, identified from screening synthetic combinatorial peptide libraries., Although all peptides bind to the same antigen combining site, the, nonhomologous peptides adopt different binding conformations and also form, their critical contacts with different antibody residues. Only small, readjustments are observed within the framework of the Fab fragment upon, binding.
+The X-ray crystal structures of an anti-p24 (HIV-1) monoclonal antibody Fab fragment alone and in complexes with the epitope peptide GATPQDLNTnL (n = norleucine), an epitope-homologous peptide GATPEDLNQKLAGN, as well as two unrelated peptides GLYEWGGARITNTD and efslkGpllqwrsG (D-peptide), are presented to a maximum resolution of 2.6 A. The latter three peptides were identified from screening synthetic combinatorial peptide libraries. Although all peptides bind to the same antigen combining site, the nonhomologous peptides adopt different binding conformations and also form their critical contacts with different antibody residues. Only small readjustments are observed within the framework of the Fab fragment upon binding.
 ==About this Structure==
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 [[Category: polyspecificity]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:35:40 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:05:41 2008''