3rsk: Difference between revisions
New page: left|200px<br /> <applet load="3rsk" size="450" color="white" frame="true" align="right" spinBox="true" caption="3rsk, resolution 2.0Å" /> '''STRUCTURE OF THE K7A... |
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==About this Structure== | ==About this Structure== | ||
3RSK is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with ACT as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3RSK OCA]]. | 3RSK is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with ACT as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5]]. Structure known Active Sites: B1, B2, P1 and P2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3RSK OCA]]. | ||
==Reference== | ==Reference== | ||
Coulombic effects of remote subsites on the active site of ribonuclease A., Fisher BM, Schultz LW, Raines RT, Biochemistry. 1998 Dec 15;37(50):17386-401. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9860854 9860854] | Coulombic effects of remote subsites on the active site of ribonuclease A., Fisher BM, Schultz LW, Raines RT, Biochemistry. 1998 Dec 15;37(50):17386-401. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9860854 9860854] | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Pancreatic ribonuclease]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Fisher, B.M.]] | [[Category: Fisher, B.M.]] | ||
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[[Category: x-ray diffraction]] | [[Category: x-ray diffraction]] | ||
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Revision as of 14:40, 30 October 2007
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STRUCTURE OF THE K7A/R10A/K66A VARIANT OF RIBONUCLEASE A
OverviewOverview
The active-site cleft of bovine pancreatic ribonuclease A (RNase A) is, lined with cationic residues that interact with a bound nucleic acid., Those residues interacting with the phosphoryl groups comprise the P0, P1, and P2 subsites, with the scissile P-O5' bond residing in the P1 subsite., Coulombic interactions between the P0 and P2 subsites and phosphoryl, groups of the substrate were characterized previously [Fisher, B. M., Ha, J.-H., and Raines, R. T. (1998) Biochemistry 37, 12121-12132]. Here, the, interactions between these subsites and the active-site residues His12 and, His119 are described in detail. A protein variant in which the cationic, residues in these subsites (Lys66 in the P0 subsite and Lys7 and Arg10 in, the P2 subsite) were replaced with alanine was crystallized, ... [(full description)]
About this StructureAbout this Structure
3RSK is a [Single protein] structure of sequence from [Bos taurus] with ACT as [ligand]. Active as [Pancreatic ribonuclease], with EC number [3.1.27.5]. Structure known Active Sites: B1, B2, P1 and P2. Full crystallographic information is available from [OCA].
ReferenceReference
Coulombic effects of remote subsites on the active site of ribonuclease A., Fisher BM, Schultz LW, Raines RT, Biochemistry. 1998 Dec 15;37(50):17386-401. PMID:9860854
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