2cjb: Difference between revisions
New page: left|200px<br /> <applet load="2cjb" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cjb, resolution 2.70Å" /> '''CRYSTAL STRUCTURE O... |
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==About this Structure== | ==About this Structure== | ||
2CJB is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri]] with ZN, CL and SER as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.11 6.1.1.11]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CJB OCA]]. | 2CJB is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri]] with ZN, CL and SER as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Serine--tRNA_ligase Serine--tRNA ligase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.11 6.1.1.11]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CJB OCA]]. | ||
==Reference== | ==Reference== | ||
Structure of the unusual seryl-tRNA synthetase reveals a distinct zinc-dependent mode of substrate recognition., Bilokapic S, Maier T, Ahel D, Gruic-Sovulj I, Soll D, Weygand-Durasevic I, Ban N, EMBO J. 2006 Jun 7;25(11):2498-509. Epub 2006 May 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16675947 16675947] | Structure of the unusual seryl-tRNA synthetase reveals a distinct zinc-dependent mode of substrate recognition., Bilokapic S, Maier T, Ahel D, Gruic-Sovulj I, Soll D, Weygand-Durasevic I, Ban N, EMBO J. 2006 Jun 7;25(11):2498-509. Epub 2006 May 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16675947 16675947] | ||
[[Category: Methanosarcina barkeri]] | [[Category: Methanosarcina barkeri]] | ||
[[Category: Serine--tRNA ligase]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ahel, D.]] | [[Category: Ahel, D.]] | ||
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[[Category: zinc ion]] | [[Category: zinc ion]] | ||
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Revision as of 14:39, 30 October 2007
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CRYSTAL STRUCTURE OF METHANOSARCINA BARKERI SERYL-TRNA SYNTHETASE COMPLEXED WITH SERINE
OverviewOverview
Methanogenic archaea possess unusual seryl-tRNA synthetase (SerRS), evolutionarily distinct from the SerRSs found in other archaea, eucaryotes, and bacteria. The two types of SerRSs show only minimal sequence, similarity, primarily within class II conserved motifs 1, 2 and 3. Here, we report a 2.5 A resolution crystal structure of the atypical, methanogenic Methanosarcina barkeri SerRS and its complexes with ATP, serine and the nonhydrolysable seryl-adenylate analogue, 5'-O-(N-serylsulfamoyl)adenosine. The structures reveal two idiosyncratic, features of methanogenic SerRSs: a novel N-terminal tRNA-binding domain, and an active site zinc ion. The tetra-coordinated Zn2+ ion is bound to, three conserved protein ligands (Cys306, Glu355 and Cys461) and binds the, amino group of the serine ... [(full description)]
About this StructureAbout this Structure
2CJB is a [Single protein] structure of sequence from [Methanosarcina barkeri] with ZN, CL and SER as [ligands]. Active as [Serine--tRNA ligase], with EC number [6.1.1.11]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Structure of the unusual seryl-tRNA synthetase reveals a distinct zinc-dependent mode of substrate recognition., Bilokapic S, Maier T, Ahel D, Gruic-Sovulj I, Soll D, Weygand-Durasevic I, Ban N, EMBO J. 2006 Jun 7;25(11):2498-509. Epub 2006 May 4. PMID:16675947
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