1beu: Difference between revisions
New page: left|200px<br /> <applet load="1beu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1beu, resolution 1.9Å" /> '''TRP SYNTHASE (D60N-I... |
No edit summary |
||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
1BEU is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]] with K, IPL and PLS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BEU OCA]]. | 1BEU is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]] with K, IPL and PLS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20]]. Structure known Active Sites: NUA and NUB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BEU OCA]]. | ||
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Salmonella typhimurium]] | [[Category: Salmonella typhimurium]] | ||
[[Category: Tryptophan synthase]] | |||
[[Category: Davies, D.R.]] | [[Category: Davies, D.R.]] | ||
[[Category: Miles, E.W.]] | [[Category: Miles, E.W.]] | ||
| Line 26: | Line 27: | ||
[[Category: mutation d60n in a-subunit]] | [[Category: mutation d60n in a-subunit]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:41:48 2007'' | ||
Revision as of 14:37, 30 October 2007
|
TRP SYNTHASE (D60N-IPP-SER) WITH K+
OverviewOverview
We have investigated the role of Asp60 of the alpha-subunit in allosteric, communication between the tryptophan synthase alpha- and beta-subunits., Crystallographic and microspectrophotometric studies have been carried out, on a mutant (alpha D60N) tryptophan synthase alpha 2 beta 2 complex which, has no observable alpha-activity, but has substantial beta-activity., Single-crystal polarized absorption spectra indicate that the external, aldimine is the predominant L-serine intermediate and that the amount of, the intermediate formed is independent of pH, monovalent cations, and, allosteric effectors. The three-dimensional structure is reported for this, mutant enzyme complexed with indole 3-propanol phosphate bound to the, alpha-site and L-serine bound to the beta-site (alpha ... [(full description)]
About this StructureAbout this Structure
1BEU is a [Protein complex] structure of sequences from [Salmonella typhimurium] with K, IPL and PLS as [ligands]. Active as [Tryptophan synthase], with EC number [4.2.1.20]. Structure known Active Sites: NUA and NUB. Full crystallographic information is available from [OCA].
ReferenceReference
Cryocrystallography and microspectrophotometry of a mutant (alpha D60N) tryptophan synthase alpha 2 beta 2 complex reveals allosteric roles of alpha Asp60., Rhee S, Miles EW, Mozzarelli A, Davies DR, Biochemistry. 1998 Jul 28;37(30):10653-9. PMID:9692955
Page seeded by OCA on Tue Oct 30 13:41:48 2007