1bo0: Difference between revisions
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==Overview== | ==Overview== | ||
1H-NMR spectroscopy and analytical ultracentrifugation studies reveal that | 1H-NMR spectroscopy and analytical ultracentrifugation studies reveal that monocyte chemoattractant protein-3 (MCP-3) is a monomer. NMR solution structure shows that MCP-3 adopts an alphabeta fold similar to what is observed in structures of other known chemokines. However, MCP-3 is unique in that it does not show a propensity to form dimers. The closely related chemokines MCP-1 and MCP-2 show a monomer-dimer equilibrium in sedimentation equilibrium studies (approximately 0.2-2 mg/ml). As these proteins are present at nanomolar concentrations in vivo, the results suggest that they are monomeric at functional concentrations and that the monomer is the functionally significant form of MCP-1, MCP-2 and MCP-3. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Kim, K | [[Category: Kim, K S.]] | ||
[[Category: Kwon, D.]] | [[Category: Kwon, D.]] | ||
[[Category: Lee, D.]] | [[Category: Lee, D.]] | ||
[[Category: Sykes, B | [[Category: Sykes, B D.]] | ||
[[Category: chemotactic cytokine]] | [[Category: chemotactic cytokine]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:57:16 2008'' | ||
Revision as of 12:57, 21 February 2008
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MONOCYTE CHEMOATTRACTANT PROTEIN-3, NMR, MINIMIZED AVERAGE STRUCTURE
OverviewOverview
1H-NMR spectroscopy and analytical ultracentrifugation studies reveal that monocyte chemoattractant protein-3 (MCP-3) is a monomer. NMR solution structure shows that MCP-3 adopts an alphabeta fold similar to what is observed in structures of other known chemokines. However, MCP-3 is unique in that it does not show a propensity to form dimers. The closely related chemokines MCP-1 and MCP-2 show a monomer-dimer equilibrium in sedimentation equilibrium studies (approximately 0.2-2 mg/ml). As these proteins are present at nanomolar concentrations in vivo, the results suggest that they are monomeric at functional concentrations and that the monomer is the functionally significant form of MCP-1, MCP-2 and MCP-3.
About this StructureAbout this Structure
1BO0 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Structural characterization of a monomeric chemokine: monocyte chemoattractant protein-3., Kim KS, Rajarathnam K, Clark-Lewis I, Sykes BD, FEBS Lett. 1996 Oct 21;395(2-3):277-82. PMID:8898111
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