1bdx: Difference between revisions
New page: left|200px<br /><applet load="1bdx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bdx, resolution 6.0Å" /> '''E. COLI DNA HELICASE ... |
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[[Image:1bdx.gif|left|200px]]<br /><applet load="1bdx" size=" | [[Image:1bdx.gif|left|200px]]<br /><applet load="1bdx" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1bdx, resolution 6.0Å" /> | caption="1bdx, resolution 6.0Å" /> | ||
'''E. COLI DNA HELICASE RUVA WITH BOUND DNA HOLLIDAY JUNCTION, ALPHA CARBONS AND PHOSPHATE ATOMS ONLY'''<br /> | '''E. COLI DNA HELICASE RUVA WITH BOUND DNA HOLLIDAY JUNCTION, ALPHA CARBONS AND PHOSPHATE ATOMS ONLY'''<br /> | ||
==Overview== | ==Overview== | ||
Here we present the crystal structure of the Escherichia coli protein RuvA | Here we present the crystal structure of the Escherichia coli protein RuvA bound to a key DNA intermediate in recombination, the Holliday junction. The structure, solved by isomorphous replacement and density modification at 6 A resolution, reveals the molecular architecture at the heart of the branch migration and resolution reactions required to process Holliday intermediates into recombinant DNA molecules. It also reveals directly for the first time the structure of the Holliday junction. A single RuvA tetramer is bound to one face of a junction whose four DNA duplex arms are arranged in an open and essentially four-fold symmetric conformation. Protein-DNA contacts are mediated by two copies of a helix-hairpin-helix motif per RuvA subunit that contact the phosphate backbone in a very similar manner. The open structure of the junction stabilized by RuvA binding exposes a DNA surface that could be bound by the RuvC endonuclease to promote resolution. | ||
==About this Structure== | ==About this Structure== | ||
1BDX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | 1BDX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BDX OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Artymiuk, P | [[Category: Artymiuk, P J.]] | ||
[[Category: Hargreaves, D.]] | [[Category: Hargreaves, D.]] | ||
[[Category: Lloyd, R | [[Category: Lloyd, R G.]] | ||
[[Category: Rafferty, J | [[Category: Rafferty, J B.]] | ||
[[Category: Rice, D | [[Category: Rice, D W.]] | ||
[[Category: Sedelnikova, S | [[Category: Sedelnikova, S E.]] | ||
[[Category: branch migration]] | [[Category: branch migration]] | ||
[[Category: complex dna-binding protein/dna]] | [[Category: complex dna-binding protein/dna]] | ||
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[[Category: ruv]] | [[Category: ruv]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:54:15 2008'' | ||
