6cel: Difference between revisions
New page: left|200px<br /> <applet load="6cel" size="450" color="white" frame="true" align="right" spinBox="true" caption="6cel, resolution 1.7Å" /> '''CBH1 (E212Q) CELLOPE... |
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==About this Structure== | ==About this Structure== | ||
6CEL is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ | 6CEL is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]] with NAG and CO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91]]. Structure known Active Sites: CAT and COB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=6CEL OCA]]. | ||
==Reference== | ==Reference== | ||
High-resolution crystal structures reveal how a cellulose chain is bound in the 50 A long tunnel of cellobiohydrolase I from Trichoderma reesei., Divne C, Stahlberg J, Teeri TT, Jones TA, J Mol Biol. 1998 Jan 16;275(2):309-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9466911 9466911] | High-resolution crystal structures reveal how a cellulose chain is bound in the 50 A long tunnel of cellobiohydrolase I from Trichoderma reesei., Divne C, Stahlberg J, Teeri TT, Jones TA, J Mol Biol. 1998 Jan 16;275(2):309-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9466911 9466911] | ||
[[Category: Cellulose 1,4-beta-cellobiosidase]] | |||
[[Category: Hypocrea jecorina]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Divne, C.]] | [[Category: Divne, C.]] | ||
[[Category: Jones, T.A.]] | [[Category: Jones, T.A.]] | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
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Revision as of 14:34, 30 October 2007
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CBH1 (E212Q) CELLOPENTAOSE COMPLEX
OverviewOverview
Detailed information has been obtained, by means of protein X-ray, crystallography, on how a cellulose chain is bound in the, cellulose-binding tunnel of cellobiohydrolase I (CBHI), the major, cellulase in the hydrolysis of native, crystalline cellulose by the fungus, Trichoderma reesei. Three high-resolution crystal structures of different, catalytically deficient mutants of CBHI in complex with cellotetraose, cellopentaose and cellohexaose have been refined at 1.9, 1.7 and 1.9 A, resolution, respectively. The observed binding of cellooligomers in the, tunnel allowed unambiguous identification of ten well-defined subsites for, glucosyl units that span a length of approximately 50 A. All bound, oligomers have the same directionality and orientation, and the positions, of the glucosyl ... [(full description)]
About this StructureAbout this Structure
6CEL is a [Single protein] structure of sequence from [Hypocrea jecorina] with NAG and CO as [ligands]. Active as [Cellulose 1,4-beta-cellobiosidase], with EC number [3.2.1.91]. Structure known Active Sites: CAT and COB. Full crystallographic information is available from [OCA].
ReferenceReference
High-resolution crystal structures reveal how a cellulose chain is bound in the 50 A long tunnel of cellobiohydrolase I from Trichoderma reesei., Divne C, Stahlberg J, Teeri TT, Jones TA, J Mol Biol. 1998 Jan 16;275(2):309-25. PMID:9466911
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