Sandbox 122: Difference between revisions
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(iii) General sequence for the PY-NLS is either a hydrophobic or basic motif at the N-terminus and a R-X2-5-P-Y motif at the C-terminus. | (iii) General sequence for the PY-NLS is either a hydrophobic or basic motif at the N-terminus and a R-X2-5-P-Y motif at the C-terminus. | ||
The <scene name='Sandbox_122/Kapb2_nls_blue_magenta/4'>PY-NLS of 2H4M</scene> contains a hydrophobic rather than a basic N- terminal motif. Hydrophobic interactions at the N- terminal motif of the PY- NLS include: <scene name='Sandbox_122/Kapb2_nls_magenta_hydrophobics/1'>Phe273, Gly274, Pro275 and Met276 </scene>of the NLS with Trp730 and Ile773 of Kapβ2. Interactions at the C- terminal R-X2-5-P-Y motif of the NLS include: Arg284 of the NLS with Glu509 and Asp543 of Kapβ2; Pro288 and Tyr289 of the NLS with Ala380, Ala 381, Ala 384, Leu419, Ile457, Trp460 and Arg464 of Kapβ2. | The <scene name='Sandbox_122/Kapb2_nls_blue_magenta/4'>PY-NLS of 2H4M</scene> contains a hydrophobic rather than a basic N- terminal motif. Hydrophobic interactions at the N- terminal motif of the PY- NLS include: <scene name='Sandbox_122/Kapb2_nls_magenta_hydrophobics/1'>Phe273, Gly274, Pro275 and Met276 </scene>of the NLS with Trp730 and Ile773 of Kapβ2. Interactions at the C- terminal R-X2-5-P-Y motif of the NLS include: <scene name='Sandbox_122/Kapb2_nls_arg284/1'>Arg284</scene>of the NLS with Glu509 and Asp543 of Kapβ2; Pro288 and Tyr289 of the NLS with Ala380, Ala 381, Ala 384, Leu419, Ile457, Trp460 and Arg464 of Kapβ2. | ||
Upon binding Kapβ2, the NLS gains structure, conforms to and makes contact with the internal surface of the Kapβ2 C-terminal arch. | Upon binding Kapβ2, the NLS gains structure, conforms to and makes contact with the internal surface of the Kapβ2 C-terminal arch. | ||