1bb6: Difference between revisions
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==Overview== | ==Overview== | ||
Two complexes between rainbow trout lysozyme (RBTL) and | Two complexes between rainbow trout lysozyme (RBTL) and 4-methylumbelliferyl chitobioside, 4MeU-(GlcNAc)2, and chitotrioside, 4MeU-(GlcNAc)3, were produced by co-crystallization and soaking, respectively, and the crystal structures were solved at 2.0 A resolution. The results show that 4-MeU-(GlcNAc)3 binds in subsites A-D and that 4-MeU-(GlcNAc)2 binds in subsites B-D in the active-site cleft of RBTL. This agrees well with earlier crystallographic studies on the binding of oligosaccharides of chitin to RBTL, which showed that (GlcNAc)3 binds to sites B-D in RBTL and not to A-C as seen in the human and turkey egg-white lysozymes. For both complexes the 4-MeU moiety in site D has diffuse electron density and is flexible, as it is only bound to water molecules and not to the protein. Since no electron density was observed in site E, the solved structures give views of nonproductive enzyme-substrate complexes. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Hough, E.]] | [[Category: Hough, E.]] | ||
[[Category: Karlsen, S.]] | [[Category: Karlsen, S.]] | ||
[[Category: Vollan, V | [[Category: Vollan, V B.]] | ||
[[Category: UMG]] | [[Category: UMG]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
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[[Category: umbelliferone glycosides]] | [[Category: umbelliferone glycosides]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:53:26 2008'' | ||
Revision as of 12:53, 21 February 2008
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LYSOZYME COMPLEX WITH 4-METHYL-UMBELLIFERYL CHITOTRIOSE
OverviewOverview
Two complexes between rainbow trout lysozyme (RBTL) and 4-methylumbelliferyl chitobioside, 4MeU-(GlcNAc)2, and chitotrioside, 4MeU-(GlcNAc)3, were produced by co-crystallization and soaking, respectively, and the crystal structures were solved at 2.0 A resolution. The results show that 4-MeU-(GlcNAc)3 binds in subsites A-D and that 4-MeU-(GlcNAc)2 binds in subsites B-D in the active-site cleft of RBTL. This agrees well with earlier crystallographic studies on the binding of oligosaccharides of chitin to RBTL, which showed that (GlcNAc)3 binds to sites B-D in RBTL and not to A-C as seen in the human and turkey egg-white lysozymes. For both complexes the 4-MeU moiety in site D has diffuse electron density and is flexible, as it is only bound to water molecules and not to the protein. Since no electron density was observed in site E, the solved structures give views of nonproductive enzyme-substrate complexes.
About this StructureAbout this Structure
1BB6 is a Single protein structure of sequence from Oncorhynchus mykiss with as ligand. Active as Lysozyme, with EC number 3.2.1.17 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Structural studies on the binding of 4-methylumbelliferone glycosides of chitin to rainbow trout lysozyme., Vollan VB, Hough E, Karlsen S, Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):60-6. Epub 1999 Jan, 1. PMID:10089395
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