1b8z: Difference between revisions
New page: left|200px<br /><applet load="1b8z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b8z, resolution 1.6Å" /> '''HU FROM THERMOTOGA MA... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1b8z.jpg|left|200px]]<br /><applet load="1b8z" size=" | [[Image:1b8z.jpg|left|200px]]<br /><applet load="1b8z" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1b8z, resolution 1.6Å" /> | caption="1b8z, resolution 1.6Å" /> | ||
'''HU FROM THERMOTOGA MARITIMA'''<br /> | '''HU FROM THERMOTOGA MARITIMA'''<br /> | ||
==Overview== | ==Overview== | ||
The humar gene encoding for the histone-like DNA-binding protein HU from | The humar gene encoding for the histone-like DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima was efficiently overexpressed in Escherichia coli under the T7 promoter. The HU protein was purified using SP-Sepharose ion-exchange and heparin-affinity chromatography and was successfully crystallized in ammonium sulfate. The crystals were grown in the tetragonal form in space group P43 or P41 and have unit-cell dimensions a = b = 46.12, c = 77.56 A, alpha = beta = gamma = 90 degrees. The crystals diffract X-rays to 1.6 A resolution using synchrotron radiation and are suitable for determination of the HU structure at high resolution. | ||
==About this Structure== | ==About this Structure== | ||
1B8Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http:// | 1B8Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B8Z OCA]. | ||
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Thermotoga maritima]] | [[Category: Thermotoga maritima]] | ||
[[Category: Christodoulou, E.]] | [[Category: Christodoulou, E.]] | ||
[[Category: Rypniewski, W | [[Category: Rypniewski, W R.]] | ||
[[Category: Vorgias, C | [[Category: Vorgias, C E.]] | ||
[[Category: thermostable dna binding protein]] | [[Category: thermostable dna binding protein]] | ||
[[Category: thermotoga maritima]] | [[Category: thermotoga maritima]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:52:52 2008'' | ||
