1azk: Difference between revisions

New page: left|200px<br /><applet load="1azk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1azk" /> '''THREE-DIMENSIONAL STRUCTURES OF THREE ENGINE...
 
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'''THREE-DIMENSIONAL STRUCTURES OF THREE ENGINEERED CELLULOSE-BINDING DOMAINS OF CELLOBIOHYDROLASE I FROM TRICHODERMA REESEI, NMR, 19 STRUCTURES'''<br />
'''THREE-DIMENSIONAL STRUCTURES OF THREE ENGINEERED CELLULOSE-BINDING DOMAINS OF CELLOBIOHYDROLASE I FROM TRICHODERMA REESEI, NMR, 19 STRUCTURES'''<br />


==Overview==
==Overview==
Three-dimensional solution structures for three engineered, synthetic CBDs, (Y5A, Y31A, and Y32A) of cellobiohydrolase I (CBHI) from Trichoderma, reesei were studied with nuclear magnetic resonance (NMR) and circular, dichroism (CD) spectroscopy. According to CD measurements the antiparallel, beta-sheet structure of the CBD fold was preserved in all engineered, peptides. The three-dimensional NMR-based structures of Y31A and Y32A, revealed only small local changes due to mutations in the flat face of, CBD, which is expected to bind to crystalline cellulose. Therefore, the, structural roles of Y31 and Y32 are minor, but their functional importance, is obvious because these mutants do not bind strongly to cellulose. In the, case of Y5A, the disruption of the structural framework at the N-terminus, and the complete loss of binding affinity implies that Y5 has both, structural and functional significance. The number of aromatic residues, and their precise spatial arrangement in the flat face of the type I CBD, fold appears to be critical for specific binding. A model for the CBD, binding in which the three aligned aromatic rings stack onto every other, glucose ring of the cellulose polymer is discussed.
Three-dimensional solution structures for three engineered, synthetic CBDs (Y5A, Y31A, and Y32A) of cellobiohydrolase I (CBHI) from Trichoderma reesei were studied with nuclear magnetic resonance (NMR) and circular dichroism (CD) spectroscopy. According to CD measurements the antiparallel beta-sheet structure of the CBD fold was preserved in all engineered peptides. The three-dimensional NMR-based structures of Y31A and Y32A revealed only small local changes due to mutations in the flat face of CBD, which is expected to bind to crystalline cellulose. Therefore, the structural roles of Y31 and Y32 are minor, but their functional importance is obvious because these mutants do not bind strongly to cellulose. In the case of Y5A, the disruption of the structural framework at the N-terminus and the complete loss of binding affinity implies that Y5 has both structural and functional significance. The number of aromatic residues and their precise spatial arrangement in the flat face of the type I CBD fold appears to be critical for specific binding. A model for the CBD binding in which the three aligned aromatic rings stack onto every other glucose ring of the cellulose polymer is discussed.


==About this Structure==
==About this Structure==
1AZK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Active as [http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AZK OCA].  
1AZK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Active as [http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AZK OCA].  


==Reference==
==Reference==
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[[Category: Hypocrea jecorina]]
[[Category: Hypocrea jecorina]]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Mattinen, M.L.]]
[[Category: Mattinen, M L.]]
[[Category: cellulase]]
[[Category: cellulase]]
[[Category: cellulose degradation]]
[[Category: cellulose degradation]]
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[[Category: protein-carbohydrate interaction]]
[[Category: protein-carbohydrate interaction]]


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