1ajy: Difference between revisions

Revision as of 12:45, 21 February 2008

STRUCTURE AND MOBILITY OF THE PUT3 DIMER: A DNA PINCER, NMR, 13 STRUCTURES

OverviewOverview

The solution structure and backbone dynamics of the transcriptional activator PUT3 (31-100) has been characterized using NMR spectroscopy. PUT3 (31-100) contains three distinct domains: a cysteine zinc cluster, linker, and dimerization domain. The cysteine zinc cluster of PUT3 closely resembles the solution structure of GAL4, while the dimerization domain forms a long coiled-coil similar to that observed in the crystal structures of GAL4 and PPR1. However, the residues at the N-terminal end of the coiled-coil behave very differently in each of these proteins. A comparison of the structural elements within this region provides a model for the DNA binding specificity of these proteins. Furthermore, we have characterized the dynamics of PUT3 to find that the zinc cluster and dimerization domains have very diverse dynamics in solution. The dimerization domain behaves as a large protein, while the peripheral cysteine zinc clusters have dynamic properties similar to small proteins.

About this StructureAbout this Structure

1AJY is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Structure and mobility of the PUT3 dimer., Walters KJ, Dayie KT, Reece RJ, Ptashne M, Wagner G, Nat Struct Biol. 1997 Sep;4(9):744-50. PMID:9303003

Page seeded by OCA on Thu Feb 21 11:45:20 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1ajy.gif|left|200px]]<br /><applet load="1ajy" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ajy.gif|left|200px]]<br /><applet load="1ajy" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ajy" />
 '''STRUCTURE AND MOBILITY OF THE PUT3 DIMER: A DNA PINCER, NMR, 13 STRUCTURES'''<br />
 ==Overview==
-The solution structure and backbone dynamics of the transcriptional, activator PUT3 (31-100) has been characterized using NMR spectroscopy., PUT3 (31-100) contains three distinct domains: a cysteine zinc cluster, linker, and dimerization domain. The cysteine zinc cluster of PUT3 closely, resembles the solution structure of GAL4, while the dimerization domain, forms a long coiled-coil similar to that observed in the crystal, structures of GAL4 and PPR1. However, the residues at the N-terminal end, of the coiled-coil behave very differently in each of these proteins. A, comparison of the structural elements within this region provides a model, for the DNA binding specificity of these proteins. Furthermore, we have, characterized the dynamics of PUT3 to find that the zinc cluster and, dimerization domains have very diverse dynamics in solution. The, dimerization domain behaves as a large protein, while the peripheral, cysteine zinc clusters have dynamic properties similar to small proteins.
+The solution structure and backbone dynamics of the transcriptional activator PUT3 (31-100) has been characterized using NMR spectroscopy. PUT3 (31-100) contains three distinct domains: a cysteine zinc cluster, linker, and dimerization domain. The cysteine zinc cluster of PUT3 closely resembles the solution structure of GAL4, while the dimerization domain forms a long coiled-coil similar to that observed in the crystal structures of GAL4 and PPR1. However, the residues at the N-terminal end of the coiled-coil behave very differently in each of these proteins. A comparison of the structural elements within this region provides a model for the DNA binding specificity of these proteins. Furthermore, we have characterized the dynamics of PUT3 to find that the zinc cluster and dimerization domains have very diverse dynamics in solution. The dimerization domain behaves as a large protein, while the peripheral cysteine zinc clusters have dynamic properties similar to small proteins.
 ==About this Structure==
-AJY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AJY OCA].
+AJY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AJY OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Saccharomyces cerevisiae]]
 [[Category: Single protein]]
-[[Category: Dayie, K.T.]]
+[[Category: Dayie, K T.]]
 [[Category: Ptashne, M.]]
-[[Category: Reece, R.J.]]
+[[Category: Reece, R J.]]
 [[Category: Wagner, G.]]
-[[Category: Walters, K.J.]]
+[[Category: Walters, K J.]]
 [[Category: ZN]]
 [[Category: put3]]
 [[Category: transcription regulation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:54:43 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:45:20 2008''