1w05: Difference between revisions
New page: left|200px<br /> <applet load="1w05" size="450" color="white" frame="true" align="right" spinBox="true" caption="1w05, resolution 2.46Å" /> '''ISOPENICILLIN N SYN... |
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==About this Structure== | ==About this Structure== | ||
1W05 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]] with FE2, SO4 and W05 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.21.3.1 1.21.3.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W05 OCA]]. | 1W05 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]] with FE2, SO4 and W05 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Isopenicillin-N_synthase Isopenicillin-N synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.21.3.1 1.21.3.1]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W05 OCA]]. | ||
==Reference== | ==Reference== | ||
Structural studies on the reaction of isopenicillin N synthase with the truncated substrate analogues delta-(L-alpha-aminoadipoyl)-L-cysteinyl-glycine and delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alanine., Long AJ, Clifton IJ, Roach PL, Baldwin JE, Rutledge PJ, Schofield CJ, Biochemistry. 2005 May 3;44(17):6619-28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15850395 15850395] | Structural studies on the reaction of isopenicillin N synthase with the truncated substrate analogues delta-(L-alpha-aminoadipoyl)-L-cysteinyl-glycine and delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alanine., Long AJ, Clifton IJ, Roach PL, Baldwin JE, Rutledge PJ, Schofield CJ, Biochemistry. 2005 May 3;44(17):6619-28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15850395 15850395] | ||
[[Category: Emericella nidulans]] | [[Category: Emericella nidulans]] | ||
[[Category: Isopenicillin-N synthase]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Clifton, I.J.]] | [[Category: Clifton, I.J.]] | ||
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[[Category: penicillin biosynthesis]] | [[Category: penicillin biosynthesis]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:32:05 2007'' | ||
Revision as of 14:27, 30 October 2007
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ISOPENICILLIN N SYNTHASE AMINOADIPOYL-CYSTEINYL-ALANINE-FE COMPLEX
OverviewOverview
Isopenicillin N synthase (IPNS), a non-heme iron(II)-dependent oxidase, catalyzes conversion of the tripeptide, delta-(l-alpha-aminoadipoyl)-l-cysteinyl-d-valine (ACV) to bicyclic, isopenicillin N (IPN), concomitant with the reduction of dioxygen to two, molecules of water. Incubation of the "truncated"substrate analogues, delta-(l-alpha-aminoadipoyl)-l-cysteinyl-glycine (ACG) and, delta-(l-alpha-aminoadipoyl)-l-cysteinyl-d-alanine (ACA) with IPNS has, previously been shown to afford acyclic products, in which the substrate, cysteinyl residue has undergone a two-electron oxidation. We report X-ray, crystal structures for the anaerobic IPNS/Fe(II)/ACG and IPNS/Fe(II)/ACA, complexes, both in the absence and presence of the dioxygen analogue, nitric oxide. The overall protein structures are ... [(full description)]
About this StructureAbout this Structure
1W05 is a [Single protein] structure of sequence from [Emericella nidulans] with FE2, SO4 and W05 as [ligands]. Active as [Isopenicillin-N synthase], with EC number [1.21.3.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Structural studies on the reaction of isopenicillin N synthase with the truncated substrate analogues delta-(L-alpha-aminoadipoyl)-L-cysteinyl-glycine and delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alanine., Long AJ, Clifton IJ, Roach PL, Baldwin JE, Rutledge PJ, Schofield CJ, Biochemistry. 2005 May 3;44(17):6619-28. PMID:15850395
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