1a8z: Difference between revisions
New page: left|200px<br /><applet load="1a8z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a8z, resolution 2.1Å" /> '''STRUCTURE DETERMINATI... |
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[[Image:1a8z.gif|left|200px]]<br /><applet load="1a8z" size=" | [[Image:1a8z.gif|left|200px]]<br /><applet load="1a8z" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1a8z, resolution 2.1Å" /> | caption="1a8z, resolution 2.1Å" /> | ||
'''STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS'''<br /> | '''STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS'''<br /> | ||
==Overview== | ==Overview== | ||
The structure of rusticyanin, an acid-stable copper protein, has been | The structure of rusticyanin, an acid-stable copper protein, has been determined at 2.1 A resolution by direct methods combined with the single-wavelength anomalous scattering (SAS) of copper (f" = 3.9 e-) and then conventionally refined (Rcryst = 18.7%, Rfree = 21.9%). This is the largest unknown protein structure (Mr approximately /= 16.8 kDa) to be determined using the SAS and direct-methods approach and demonstrates that by exploiting the anomalous signal at a single wavelength, direct methods can be used to determine phases at typical (approximately 2 A) macromolecular crystallographic resolutions. Extrapolating from the size of the anomalous signal for copper (f" approximately 4 e-), this result suggests that the approach could be used for proteins with molecular weights of up to 33 kDa per Se (f"max++ = 8 e- at the 'white line') and 80 kDa for a Pt derivative (f"max = 19 e- at the 'white line', L3 edge). The method provides a powerful alternative in solving a de novo protein structure without either preparing multiple crystals (i.e. isomorphous heavy-atom derivative plus native crystals) or collecting multi-wavelength anomalous diffraction (MAD) data. | ||
==About this Structure== | ==About this Structure== | ||
1A8Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans] with CU1 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1A8Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans] with <scene name='pdbligand=CU1:'>CU1</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A8Z OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Acidithiobacillus ferrooxidans]] | [[Category: Acidithiobacillus ferrooxidans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Duke, E | [[Category: Duke, E M.H.]] | ||
[[Category: Hao, Q.]] | [[Category: Hao, Q.]] | ||
[[Category: Harvey, I.]] | [[Category: Harvey, I.]] | ||
[[Category: Hasnain, S | [[Category: Hasnain, S S.]] | ||
[[Category: Ingledew, W | [[Category: Ingledew, W J.]] | ||
[[Category: CU1]] | [[Category: CU1]] | ||
[[Category: copper protein]] | [[Category: copper protein]] | ||
| Line 26: | Line 26: | ||
[[Category: sas]] | [[Category: sas]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:42:10 2008'' | ||
Revision as of 12:42, 21 February 2008
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STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS
OverviewOverview
The structure of rusticyanin, an acid-stable copper protein, has been determined at 2.1 A resolution by direct methods combined with the single-wavelength anomalous scattering (SAS) of copper (f" = 3.9 e-) and then conventionally refined (Rcryst = 18.7%, Rfree = 21.9%). This is the largest unknown protein structure (Mr approximately /= 16.8 kDa) to be determined using the SAS and direct-methods approach and demonstrates that by exploiting the anomalous signal at a single wavelength, direct methods can be used to determine phases at typical (approximately 2 A) macromolecular crystallographic resolutions. Extrapolating from the size of the anomalous signal for copper (f" approximately 4 e-), this result suggests that the approach could be used for proteins with molecular weights of up to 33 kDa per Se (f"max++ = 8 e- at the 'white line') and 80 kDa for a Pt derivative (f"max = 19 e- at the 'white line', L3 edge). The method provides a powerful alternative in solving a de novo protein structure without either preparing multiple crystals (i.e. isomorphous heavy-atom derivative plus native crystals) or collecting multi-wavelength anomalous diffraction (MAD) data.
About this StructureAbout this Structure
1A8Z is a Single protein structure of sequence from Acidithiobacillus ferrooxidans with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Structure determination of a 16.8 kDa copper protein at 2.1 A resolution using anomalous scattering data with direct methods., Harvey I, Hao Q, Duke EM, Ingledew WJ, Hasnain SS, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):629-35. PMID:9761859
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