1a8q: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
The structures of cofactor-free haloperoxidases from Streptomyces | The structures of cofactor-free haloperoxidases from Streptomyces aureofaciens, Streptomyces lividans, and Pseudomonas fluorescens have been determined at resolutions between 1.9 A and 1.5 A. The structures of two enzymes complexed with benzoate or propionate identify the binding site for the organic acids which are required for the haloperoxidase activity. Based on these complexes and on the structure of an inactive variant, a reaction mechanism is proposed for the halogenation reaction with peroxoacid and hypohalous acid as reaction intermediates. Comparison of the structures suggests that a specific halide binding site is absent in the enzymes but that hydrophobic organic compounds may fit into the active site pocket for halogenation at preferential sites. | ||
==About this Structure== | ==About this Structure== | ||
| Line 15: | Line 15: | ||
[[Category: Streptomyces aureofaciens]] | [[Category: Streptomyces aureofaciens]] | ||
[[Category: Altenbuchner, J.]] | [[Category: Altenbuchner, J.]] | ||
[[Category: Hecht, H | [[Category: Hecht, H J.]] | ||
[[Category: Hofmann, B.]] | [[Category: Hofmann, B.]] | ||
[[Category: Pee, K | [[Category: Pee, K H.Van.]] | ||
[[Category: Pelletier, I.]] | [[Category: Pelletier, I.]] | ||
[[Category: Toelzer, S.]] | [[Category: Toelzer, S.]] | ||
| Line 23: | Line 23: | ||
[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:42:06 2008'' | ||