Sandbox 122: Difference between revisions
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HEAT repeats 9-13 and 14-18 form the binding site of Kapβ2 cargos while repeats 1-8 constitute the Ran GTPase binding site.3 Ran GTPase, a small 216-residue protein, is found more frequently in the nucleus and enables cargos to be released from KapB2. | HEAT repeats 9-13 and 14-18 form the binding site of Kapβ2 cargos while repeats 1-8 constitute the Ran GTPase binding site.3 Ran GTPase, a small 216-residue protein, is found more frequently in the nucleus and enables cargos to be released from KapB2. | ||
Kapβ2 Cargo Binding and Conformational Change4 | ===Kapβ2 Cargo Binding and Conformational Change4=== | ||
The NLSs located on Kapβ2 cargos are named the PY- NLS and they bind to the C-terminal arch of Kapβ2. | The NLSs located on Kapβ2 cargos are named the PY- NLS and they bind to the C-terminal arch of Kapβ2. | ||
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Upon binding Kapβ2, the NLS gains structure, conforms to and makes contact with the internal surface of the Kapβ2 C-terminal arch. | Upon binding Kapβ2, the NLS gains structure, conforms to and makes contact with the internal surface of the Kapβ2 C-terminal arch. | ||
'''How does Kapβ2 pass through the NPC?''' | ==='''How does Kapβ2 pass through the NPC?'''=== | ||
Once the cargo binds to Kapβ2, the complex travels through the NPC by interactions with the FG-Nups. | Once the cargo binds to Kapβ2, the complex travels through the NPC by interactions with the FG-Nups. | ||