Sandbox 122: Difference between revisions
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The PY-NLS of 2H4M contains a hydrophobic rather than a basic N- terminal motif. Hydrophobic interactions at the N- terminal motif of the PY- NLS include: Phe273, Gly274, Pro275 and Met276 of the NLS with Trp730 and Ile773 of Kapβ2. Interactions at the C- terminal R-X2-5-P-Y motif of the NLS include: Arg284 of the NLS with Glu509 and Asp543 of Kapβ2; Pro288 and Tyr289 of the NLS with Ala380, Ala 381, Ala 384, Leu419, Ile457, Trp460 and Arg464 of Kapβ2. | The PY-NLS of 2H4M contains a hydrophobic rather than a basic N- terminal motif. Hydrophobic interactions at the N- terminal motif of the PY- NLS include: Phe273, Gly274, Pro275 and Met276 of the NLS with Trp730 and Ile773 of Kapβ2. Interactions at the C- terminal R-X2-5-P-Y motif of the NLS include: Arg284 of the NLS with Glu509 and Asp543 of Kapβ2; Pro288 and Tyr289 of the NLS with Ala380, Ala 381, Ala 384, Leu419, Ile457, Trp460 and Arg464 of Kapβ2. | ||
Upon | Upon binding Kapβ2, the NLS gains structure, conforms to and makes contact with the internal surface of the Kapβ2 C-terminal arch. | ||
'''How does Kapβ2 pass through the NPC?''' | '''How does Kapβ2 pass through the NPC?''' | ||