Hox protein: Difference between revisions

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Jaime Prilusky, Remo Rohs, Bailey Holmes, Ana Carolina Dantas Machado, Iris Dror, Keziah Kim, Sharon Kim, Julia Tam, Skyler Saleebyan, Masha Karelina, Eric Martz, Alexander Berchansky, Michal Harel, Angel Herraez, Joel L. Sussman

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 [[Image:Joshi-etal-Figure7.jpg‎ |thumb|left|300px|Figure 4: Expression patterns of Scr in presence of Scr specific site (left panel) vs. Hox consensus site (right panel). Elsevier/Cell Press has provided permission for usage of this figure<ref name="joshi"/>.]]
-In vitro binding studies have shown that His-12 and Arg3 mutations have a large effect when exposed to the Scr specific site, whereas the effect is small when exposed to a Hox consensus site. The biological importance of both side chains becomes apparent in in-vivo experiments. Upon mutations of His-12 and Arg3 to alanine, Scr expression in a fly embryo is dramatically affected (Figure 4). In comparison to  wild type Scr (A) and based on ectopic expression (B), there is only residual expression detected in
+In vitro binding studies have shown that His-12 and Arg3 mutations have a large effect when exposed to the Scr specific site, whereas the effect is small when exposed to a Hox consensus site. The biological importance of both side chains becomes apparent in ''in vivo'' experiments. Upon mutations of His-12 and Arg3 to alanine, Scr expression in a fly embryo is dramatically affected (Figure 4). In comparison to  wild type Scr (A) and based on ectopic expression (B), there is only residual expression detected in
 the thorax region of the double mutant when the Scr specific site is tested (C), whereas there is no apparent effect on expression in the presence of a Hox consensus site (D-F).<br/>