197d: Difference between revisions
New page: left|200px<br /><applet load="197d" size="450" color="white" frame="true" align="right" spinBox="true" caption="197d, resolution 2.190Å" /> '''ORTHORHOMBIC CRYSTA... |
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[[Image:197d.gif|left|200px]]<br /><applet load="197d" size=" | [[Image:197d.gif|left|200px]]<br /><applet load="197d" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="197d, resolution 2.190Å" /> | caption="197d, resolution 2.190Å" /> | ||
'''ORTHORHOMBIC CRYSTAL STRUCTURE OF THE A-DNA OCTAMER D(GTACGTAC). COMPARISON WITH THE TETRAGONAL STRUCTURE'''<br /> | '''ORTHORHOMBIC CRYSTAL STRUCTURE OF THE A-DNA OCTAMER D(GTACGTAC). COMPARISON WITH THE TETRAGONAL STRUCTURE'''<br /> | ||
==Overview== | ==Overview== | ||
The X-ray crystal structure of the double-helical A-DNA octanucleotide | The X-ray crystal structure of the double-helical A-DNA octanucleotide d(GTACGTAC) has been solved by molecular replacement and refined to a resolution of 0.219 nm. The final R-factor is equal to 16.1% for 1516 observed reflections with F > 4 sigma(F). The sequence crystallizes as an A-DNA-type double helix in the orthorhombic space group P2(1)2(1)2, with one duplex molecule solvated by 66 water molecules in the asymmetric unit. Cell parameters are a = 3.860 nm, b = 5.082 nm, c = 2.174 nm. It is the first time that such a crystal form has been observed. This orthorhombic structure has been compared with the tetragonal structure of the same oligonucleotide. It adopts a bent structure with an unusual packing between symmetry-related molecules. | ||
==About this Structure== | ==About this Structure== | ||
197D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | 197D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=197D OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Courseille, C.]] | [[Category: Courseille, C.]] | ||
[[Category: Dautant, A.]] | [[Category: Dautant, A.]] | ||
[[Category: Estaintot, B | [[Category: Estaintot, B Langlois D.]] | ||
[[Category: Precigoux, G.]] | [[Category: Precigoux, G.]] | ||
[[Category: a-dna]] | [[Category: a-dna]] | ||
[[Category: double helix]] | [[Category: double helix]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:39:22 2008'' | ||
Revision as of 12:39, 21 February 2008
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ORTHORHOMBIC CRYSTAL STRUCTURE OF THE A-DNA OCTAMER D(GTACGTAC). COMPARISON WITH THE TETRAGONAL STRUCTURE
OverviewOverview
The X-ray crystal structure of the double-helical A-DNA octanucleotide d(GTACGTAC) has been solved by molecular replacement and refined to a resolution of 0.219 nm. The final R-factor is equal to 16.1% for 1516 observed reflections with F > 4 sigma(F). The sequence crystallizes as an A-DNA-type double helix in the orthorhombic space group P2(1)2(1)2, with one duplex molecule solvated by 66 water molecules in the asymmetric unit. Cell parameters are a = 3.860 nm, b = 5.082 nm, c = 2.174 nm. It is the first time that such a crystal form has been observed. This orthorhombic structure has been compared with the tetragonal structure of the same oligonucleotide. It adopts a bent structure with an unusual packing between symmetry-related molecules.
About this StructureAbout this Structure
197D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
ReferenceReference
Orthorhombic crystal structure of the A-DNA octamer d(GTACGTAC). Comparison with the tetragonal structure., Langlois d'Estaintot B, Dautant A, Courseille C, Precigoux G, Eur J Biochem. 1993 Apr 15;213(2):673-82. PMID:8477741
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