1fwh: Difference between revisions
New page: left|200px<br /> <applet load="1fwh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fwh, resolution 2.0Å" /> '''KLEBSIELLA AEROGENES... |
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==About this Structure== | ==About this Structure== | ||
1FWH is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes]] with NI as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FWH OCA]]. | 1FWH is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes]] with NI as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Urease Urease]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5]]. Structure known Active Sites: ACT and NIL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FWH OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: Klebsiella aerogenes]] | [[Category: Klebsiella aerogenes]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Urease]] | |||
[[Category: Karplus, P.A.]] | [[Category: Karplus, P.A.]] | ||
[[Category: Pearson, M.A.]] | [[Category: Pearson, M.A.]] | ||
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[[Category: nickel metalloenzyme]] | [[Category: nickel metalloenzyme]] | ||
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Revision as of 14:24, 30 October 2007
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KLEBSIELLA AEROGENES UREASE, C319Y VARIANT
OverviewOverview
Cys319 is located on a mobile flap covering the active site of Klebsiella, aerogenes urease but does not play an essential role in catalysis. Four, urease variants altered at position C319 range from having high activity, (C319A) to no measurable activity (C319Y), indicating Cys is not required, at this position, but its presence is highly influential [Martin, P. R., &, Hausinger, R. P. (1992) J. Biol. Chem. 267, 20024-20027]. Here, we present, 2.0 A resolution crystal structures of C319A, C319S, C319D, and C319Y, proteins and the C319A variant inhibited by acetohydroxamic acid. These, structures show changes in the hydration of the active site nickel ions, and in the position and flexibility of the active site flap. The C319Y, protein exhibits an alternate conformation of the flap, ... [(full description)]
About this StructureAbout this Structure
1FWH is a [Protein complex] structure of sequences from [Klebsiella aerogenes] with NI as [ligand]. Active as [Urease], with EC number [3.5.1.5]. Structure known Active Sites: ACT and NIL. Full crystallographic information is available from [OCA].
ReferenceReference
Structures of Cys319 variants and acetohydroxamate-inhibited Klebsiella aerogenes urease., Pearson MA, Michel LO, Hausinger RP, Karplus PA, Biochemistry. 1997 Jul 1;36(26):8164-72. PMID:9201965
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