Hox protein: Difference between revisions
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[[Image:Joshi-etal-Figure7.jpg |thumb|left|300px|Figure 4: Expression patterns of Scr in presence of Scr specific site (left panel) vs. Hox consensus site (right panel). Elsevier/Cell Press has provided permission for usage of this figure<ref name="joshi"/>.]] | [[Image:Joshi-etal-Figure7.jpg |thumb|left|300px|Figure 4: Expression patterns of Scr in presence of Scr specific site (left panel) vs. Hox consensus site (right panel). Elsevier/Cell Press has provided permission for usage of this figure<ref name="joshi"/>.]] | ||
<Structure load='2r5y' size='300' frame='true' align='right' caption='Figure 5: 3D-Representation of Exd-Scr-DNA ternary complex with Hox consensus site; PDB ID# 2R5Y.' scene='Sandbox_Reserved_169/Con/3' /> | <div align="right"><Structure load='2r5y' size='300' frame='true' align='right' caption='Figure 5: 3D-Representation of Exd-Scr-DNA ternary complex with Hox consensus site; PDB ID# 2R5Y.' scene='Sandbox_Reserved_169/Con/3' /></div> | ||
In vitro binding studies have shown that His-12 and Arg3 mutations have a large effect when exposed to the Scr specific site, whereas the effect is small when exposed to a Hox consensus site. In vivo experiments, the biological importance of both side chains becomes apparent as upon mutations of His-12 and Arg3 to alanine expression in a fly embryo is affected (Figure 4). In comparison to wild type Scr (A) and based on ectopic expression (B), there is only residual expression detected in the thorax region of the double mutant when the Scr specific site is tested (C), whereas there is no apparent effect on expression in the presence of a Hox consensus site (D-F).<br/> | <div align="left">In vitro binding studies have shown that His-12 and Arg3 mutations have | ||
a large effect when exposed to the Scr specific site, whereas the effect is small when exposed to a Hox consensus site. In vivo experiments, the biological importance of both side chains becomes apparent as upon mutations of His-12 and Arg3 to alanine expression in a fly embryo is affected (Figure 4). In comparison to wild type Scr (A) and based on ectopic expression (B), there is only residual expression detected in | |||
the thorax region of the double mutant when the Scr specific site is | |||
tested (C), whereas there is no apparent effect on expression in the presence of | |||
a Hox consensus site (D-F).<br/></div> | |||
==Recognition of Scr Specific vs. Hox Consensus Site== | ==Recognition of Scr Specific vs. Hox Consensus Site== | ||