2kmb: Difference between revisions
New page: left|200px<br /> <applet load="2kmb" size="450" color="white" frame="true" align="right" spinBox="true" caption="2kmb, resolution 2.0Å" /> '''COMPLEX OF 3'-NEUAC-... |
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==About this Structure== | ==About this Structure== | ||
2KMB is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]] with CA and CL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2KMB OCA]]. | 2KMB is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]] with CA and CL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Sites: 11, 12, 13, 21, 22, 23, 31, 32 and 33. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2KMB OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: lectin]] | [[Category: lectin]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:27:32 2007'' | ||
Revision as of 14:22, 30 October 2007
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COMPLEX OF 3'-NEUAC-LEWIS-X WITH A SELECTIN-LIKE MUTANT OF MANNOSE-BINDING PROTEIN A
OverviewOverview
Rat serum mannose-binding protein in which residues 211-213 have been, changed to the Lys-Lys-Lys sequence found in E-selectin binds HL-60 cells, and the oligosaccharide 3'-NeuAc-Le(x). To understand how this mutant, designated K3, mimics the carbohydrate-binding properties of E-selectin, structures of K3 alone and in complexes with 3'-NeuAc-Le(x), 3'-sulfo-Le(x) and 4'-sulfo-Le(x) have been determined at 1.95-2.1 A, resolution by X-ray crystallography. The region of K3 that interacts with, bound oligosaccharides superimposes closely with the corresponding region, of unliganded E-selectin. In each of the oligosaccharide-protein, complexes, the 2- and 3-OH of Fuc coordinate Ca2+ and form a network of, cooperative hydrogen bonds with amino acid side chains that also, coordinate the Ca2+. ... [(full description)]
About this StructureAbout this Structure
2KMB is a [Single protein] structure of sequence from [Rattus norvegicus] with CA and CL as [ligands]. Structure known Active Sites: 11, 12, 13, 21, 22, 23, 31, 32 and 33. Full crystallographic information is available from [OCA].
ReferenceReference
Structure of a selectin-like mutant of mannose-binding protein complexed with sialylated and sulfated Lewis(x) oligosaccharides., Ng KK, Weis WI, Biochemistry. 1997 Feb 4;36(5):979-88. PMID:9033386
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