1uq5: Difference between revisions
New page: left|200px<br /> <applet load="1uq5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uq5, resolution 1.4Å" /> '''RICIN A-CHAIN (RECOM... |
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==About this Structure== | ==About this Structure== | ||
1UQ5 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Ricinus_communis Ricinus communis]] with ACT and SO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UQ5 OCA]]. | 1UQ5 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Ricinus_communis Ricinus communis]] with ACT and SO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UQ5 OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: Ricinus communis]] | [[Category: Ricinus communis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: rRNA N-glycosylase]] | |||
[[Category: Fulop, V.]] | [[Category: Fulop, V.]] | ||
[[Category: Marsden, C.J.]] | [[Category: Marsden, C.J.]] | ||
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[[Category: toxin]] | [[Category: toxin]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:27:09 2007'' | ||
Revision as of 14:22, 30 October 2007
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RICIN A-CHAIN (RECOMBINANT) N122A MUTANT
OverviewOverview
Models for the binding of the sarcin-ricin loop (SRL) of 28S ribosomal RNA, to ricin A chain (RTA) suggest that several surface exposed arginine, residues surrounding the active site cleft make important interactions, with the RNA substrate. The data presented in this study suggest differing, roles for these arginyl residues. Substitution of Arg48 or Arg213 with Ala, lowered the activity of RTA 10-fold. Furthermore, substitution of Arg213, with Asp lowered the activity of RTA 100-fold. The crystal structure of, this RTA variant showed it to have an unaltered tertiary structure, suggesting that the positively charged state of Arg213 is crucial for, activity. Substitution of Arg258 with Ala had no effect on activity, although substitution with Asp lowered activity 10-fold. Substitution of, ... [(full description)]
About this StructureAbout this Structure
1UQ5 is a [Single protein] structure of sequence from [Ricinus communis] with ACT and SO4 as [ligands]. Active as [rRNA N-glycosylase], with EC number [3.2.2.22]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain., Marsden CJ, Fulop V, Day PJ, Lord JM, Eur J Biochem. 2004 Jan;271(1):153-62. PMID:14686928
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