1yef: Difference between revisions
New page: left|200px<br /> <applet load="1yef" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yef, resolution 2.0Å" /> '''STRUCTURE OF IGG2A F... |
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'''STRUCTURE OF IGG2A FAB FRAGMENT (D2.3) COMPLEXED WITH SUBSTRATE ANALOGUE'''<br /> | '''STRUCTURE OF IGG2A FAB FRAGMENT (D2.3) COMPLEXED WITH SUBSTRATE ANALOGUE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1YEF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ZN and PNC as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1YEF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=PNC:'>PNC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YEF OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: catalytic antibody]] | [[Category: catalytic antibody]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:11:40 2008'' | ||
Revision as of 18:11, 15 February 2008
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STRUCTURE OF IGG2A FAB FRAGMENT (D2.3) COMPLEXED WITH SUBSTRATE ANALOGUE
OverviewOverview
The x-ray structures of the unliganded esterase-like catalytic antibody, D2.3 and its complexes with a substrate analogue and with one of the, reaction products are analyzed. Together with the structure of the, phosphonate transition state analogue hapten complex, these crystal, structures provide a complete description of the reaction pathway. At, alkaline pH, D2.3 acts by preferential stabilization of the negatively, charged oxyanion intermediate of the reaction that results from hydroxide, attack on the substrate. A tyrosine residue plays a crucial role in, catalysis: it activates the ester substrate and, together with an, asparagine, it stabilizes the oxyanion intermediate. A canal allows facile, diffusion of water molecules to the reaction center that is deeply buried, in the structure. Residues bordering this canal provide targets for, mutagenesis to introduce a general base in the vicinity of the reaction, center.
About this StructureAbout this Structure
1YEF is a Protein complex structure of sequences from Mus musculus with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
X-ray structures of a hydrolytic antibody and of complexes elucidate catalytic pathway from substrate binding and transition state stabilization through water attack and product release., Gigant B, Charbonnier JB, Eshhar Z, Green BS, Knossow M, Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):7857-61. PMID:9223277
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