1ua6: Difference between revisions
New page: left|200px<br /> <applet load="1ua6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ua6, resolution 1.9Å" /> '''Crystal structure of... |
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'''Crystal structure of HYHEL-10 FV MUTANT SFSF complexed with HEN EGG WHITE LYSOZYME complex'''<br /> | '''Crystal structure of HYHEL-10 FV MUTANT SFSF complexed with HEN EGG WHITE LYSOZYME complex'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1UA6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | 1UA6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UA6 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: mutant]] | [[Category: mutant]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:00:27 2008'' | ||
Revision as of 18:00, 15 February 2008
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Crystal structure of HYHEL-10 FV MUTANT SFSF complexed with HEN EGG WHITE LYSOZYME complex
OverviewOverview
Decreased affinity of an antibody for a mutated epitope in an antigen can, be enhanced and reversed by mutations in certain antibody residues. Here, we describe the crystal structures of (a) the complex between a naturally, mutated proteinaceous antigen and an antibody that was mutated and, selected in vitro, and (b) the complex between the normal antigen and the, mutated antibody. The mutated and selected antibody recognizes essentially, the same epitope as in the wild-type antibody, indicating successful, target site-directed functional alteration of the antibody. In comparing, the structure of the mutated antigen-mutant antibody complex with the, previously established structure of the wild-type antigen-wild-type, antibody complex, we found that the enhanced affinity of the mutated, antibody for the mutant antigen originated not from improvements in local, complementarity around the mutated sites but from subtle and critical, structural changes in nonmutated sites, including an increase in variable, domain interactions. Our findings indicate that only a few mutations in, the antigen-binding region of an antibody can lead to some structural, changes in its paratopes, emphasizing the critical roles of the plasticity, of loops in the complementarity-determining region and also the importance, of the plasticity of the interaction between the variable regions of, immunoglobulin heavy and light chains in determining the specificity of an, antibody.
About this StructureAbout this Structure
1UA6 is a Protein complex structure of sequences from Gallus gallus and Mus musculus. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
ReferenceReference
Structural consequences of target epitope-directed functional alteration of an antibody. The case of anti-hen lysozyme antibody, HyHEL-10., Kumagai I, Nishimiya Y, Kondo H, Tsumoto K, J Biol Chem. 2003 Jul 4;278(27):24929-36. Epub 2003 Apr 22. PMID:12709438
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