1tu4: Difference between revisions
New page: left|200px<br /> <applet load="1tu4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tu4, resolution 2.20Å" /> '''Crystal Structure o... |
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[[Image:1tu4. | [[Image:1tu4.jpg|left|200px]]<br /><applet load="1tu4" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="1tu4, resolution 2.20Å" /> | caption="1tu4, resolution 2.20Å" /> | ||
'''Crystal Structure of Rab5-GDP Complex'''<br /> | '''Crystal Structure of Rab5-GDP Complex'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1TU4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CO, SO4 and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1TU4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CO:'>CO</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TU4 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: rab5]] | [[Category: rab5]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:58:30 2008'' | ||
Revision as of 17:58, 15 February 2008
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Crystal Structure of Rab5-GDP Complex
OverviewOverview
Rab5 is a small GTPase that regulates early endosome fusion. We present, here the crystal structure of the Rab5 GTPase domain in complex with a GTP, analog and the C-terminal domain of effector Rabaptin5. The proteins form, a dyad-symmetric Rab5-Rabaptin5(2)-Rab5 ternary complex with a parallel, coiled-coil Rabaptin5 homodimer in the middle. Two Rab5 molecules bind, independently to the Rabaptin5 dimer using their switch and interswitch, regions. The binding does not involve the Rab complementarity-determining, regions. We also present the crystal structures of two distinct forms of, GDP-Rab5 complexes, both of which are incompatible with Rabaptin5 binding., One has a dislocated and disordered switch I but a virtually intact switch, II, whereas the other has its beta-sheet and both switch regions, reorganized. Biochemical and functional analyses show that the, crystallographically observed Rab5-Rabaptin5 complex also exists in, solution, and disruption of this complex by mutation abrogates endosome, fusion.
About this StructureAbout this Structure
1TU4 is a Single protein structure of sequence from Homo sapiens with , and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis of Rab5-Rabaptin5 interaction in endocytosis., Zhu G, Zhai P, Liu J, Terzyan S, Li G, Zhang XC, Nat Struct Mol Biol. 2004 Oct;11(10):975-83. Epub 2004 Sep 19. PMID:15378032
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