1tev: Difference between revisions
New page: left|200px<br /> <applet load="1tev" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tev, resolution 2.10Å" /> '''Crystal structure o... |
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'''Crystal structure of the human UMP/CMP kinase in open conformation'''<br /> | '''Crystal structure of the human UMP/CMP kinase in open conformation'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1TEV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cytidylate_kinase Cytidylate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.14 2.7.4.14] Full crystallographic information is available from [http:// | 1TEV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cytidylate_kinase Cytidylate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.14 2.7.4.14] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TEV OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: ploop]] | [[Category: ploop]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:56:40 2008'' | ||
Revision as of 17:56, 15 February 2008
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Crystal structure of the human UMP/CMP kinase in open conformation
OverviewOverview
Human UMP/CMP kinase plays a crucial role in supplying precursors for, nucleic acid synthesis by catalyzing the conversion of UMP, CMP, and dCMP, into their diphosphate form. In addition, this kinase is an essential, component of the activation cascade of medicinally relevant nucleoside, analog prodrugs such as AraC, gemcitabine, and ddC. During the catalytic, cycle the enzyme undergoes large conformational changes from open in the, absence of substrates to closed in the presence of both phosphoryl donor, and phosphoryl acceptor. Here we report the crystal structure of the, substrate-free, open form of human UMP/CMP kinase. Comparison of the open, structure with the closed state previously reported for the similar, Dictyostelium discoideum UMP/CMP kinase reveals the conformational changes, that occur upon substrate binding. We observe a classic example of induced, fit where substrate-induced conformational changes in hinge residues, result in rigid body movements of functional domains to form the, catalytically competent state. In addition, a homology model of the human, enzyme in the closed state based on the structure of D. discoideum UMP/CMP, kinase aids to rationalize the substrate specificity of the human enzyme.
DiseaseDisease
Known disease associated with this structure: HIV infection, susceptibility/resistance to OMIM:[601267]
About this StructureAbout this Structure
1TEV is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Cytidylate kinase, with EC number 2.7.4.14 Full crystallographic information is available from OCA.
ReferenceReference
Substrate-induced conformational changes in human UMP/CMP kinase., Segura-Pena D, Sekulic N, Ort S, Konrad M, Lavie A, J Biol Chem. 2004 Aug 6;279(32):33882-9. Epub 2004 May 26. PMID:15163660
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