Sandbox Reserved 479: Difference between revisions
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It was not until recently that crystal structures could be used to analyze myosin and it's role in the actinomysin chemomechanical cycle. X-ray diffraction studies have been conducted on whole muscles and muscle fibers to learn how structural changes promote and facilitate motor activity. Movement of myosin was analyzed under near physiological conditions using modern synchrotron radiation sources. Structural alterations were created through use of unique time and spatial resolution. Muscle cells exhibit low scattering power in X-ray images, so a clear crystalline structure cannot not form. A flat detector is needed because reflections are concentrated in the low angle regions. Snychrotron radiation generates a monochromatic X-ray breams of diameter 0.2-0.3 mm, similar to that of the muscle fiber, and making high resolution images and video of a single muscle cell possible.<ref name="Koubassova">PMID: 22339600</ref> | It was not until recently that crystal structures could be used to analyze myosin and it's role in the actinomysin chemomechanical cycle. X-ray diffraction studies have been conducted on whole muscles and muscle fibers to learn how structural changes promote and facilitate motor activity. Movement of myosin was analyzed under near physiological conditions using modern synchrotron radiation sources. Structural alterations were created through use of unique time and spatial resolution. Muscle cells exhibit low scattering power in X-ray images, so a clear crystalline structure cannot not form. A flat detector is needed because reflections are concentrated in the low angle regions. Snychrotron radiation generates a monochromatic X-ray breams of diameter 0.2-0.3 mm, similar to that of the muscle fiber, and making high resolution images and video of a single muscle cell possible.<ref name="Koubassova">PMID: 22339600</ref> | ||
==Interaction with Actin== | ==Interaction with Actin==<ref name="esrf">[http://www.esrf.eu/UsersAndScience/Publications/Highlights/2003/MX/MX03]</ref> | ||
[[Image:actinandmyosin.png]] | [[Image:actinandmyosin.png]] | ||
==Mechanism of Action== | ==Mechanism of Action== | ||