NAC transcription factor: Difference between revisions
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== Overview == | == Overview == | ||
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Vascular-related NAC-domain transcription facor (VND)is one group of the largest plant-specific transcription factor NAC family. The VND1-VND7 were orginally isolated as genes for which expression levels are elevated during transdifferentiation into trachery elements, in a induction system using Arabidopsis suspension cells [1].In the past several years, VNDs have been intensively investigated in different species and shown to be important switches of the biosynthesis of secondary cell walls that provide textiles, timber, and potentially second-generation bio-fuels for human use[2,3]. | Vascular-related NAC-domain transcription facor (VND)is one group of the largest plant-specific transcription factor NAC family. The VND1-VND7 were orginally isolated as genes for which expression levels are elevated during transdifferentiation into trachery elements, in a induction system using Arabidopsis suspension cells [1].In the past several years, VNDs have been intensively investigated in different species and shown to be important switches of the biosynthesis of secondary cell walls that provide textiles, timber, and potentially second-generation bio-fuels for human use[2,3]. | ||
VNDs are grouped in NAC-c subfamily [4]. Typically, the proteins in this subfamily share a well conserved N-terminal NAC domain (-150 amino acid;aa) and a diversified C-terminal transcription regulatory region [5,6]. The N-terminal NAC domain is usually responsible for DNA binding and dimerization, and the C-terminal region function in transcription activation , repression and protein binding. X-ray crystallography have exhibited the structure of conserved NAC domains when they form dimer and bind with DNA. However, due to the diversified sequence of C-terminal region, no structure analyses have been conducted in the region. | VNDs are grouped in NAC-c subfamily [4]. Typically, the proteins in this subfamily share a well conserved N-terminal NAC domain (-150 amino acid;aa) and a diversified C-terminal transcription regulatory region [5,6]. The N-terminal NAC domain is usually responsible for DNA binding and dimerization, and the C-terminal region function in transcription activation , repression and protein binding. X-ray crystallography have exhibited the structure of conserved NAC domains when they form dimer and bind with DNA. However, due to the diversified sequence of C-terminal region, no structure analyses have been conducted in the region. | ||
Revision as of 00:30, 2 May 2012
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Vascular-related NAC-domain transcription facor (VND)is one group of the largest plant-specific transcription factor NAC family. The VND1-VND7 were orginally isolated as genes for which expression levels are elevated during transdifferentiation into trachery elements, in a induction system using Arabidopsis suspension cells [1].In the past several years, VNDs have been intensively investigated in different species and shown to be important switches of the biosynthesis of secondary cell walls that provide textiles, timber, and potentially second-generation bio-fuels for human use[2,3]. VNDs are grouped in NAC-c subfamily [4]. Typically, the proteins in this subfamily share a well conserved N-terminal NAC domain (-150 amino acid;aa) and a diversified C-terminal transcription regulatory region [5,6]. The N-terminal NAC domain is usually responsible for DNA binding and dimerization, and the C-terminal region function in transcription activation , repression and protein binding. X-ray crystallography have exhibited the structure of conserved NAC domains when they form dimer and bind with DNA. However, due to the diversified sequence of C-terminal region, no structure analyses have been conducted in the region.
The NAC domainThe NAC domain
The DNA binding activity of NAC proteins is restricted into NAC domain which was divided into five subdomains A-E. The highly conserved positively charged subdomains C and D bind to DNA, whereas subdomain A may be involved in the formation of a functional dimer. X-ray crystallograhy have exhibited the presence of a novel transcription factor fold consisting of a twirled antiparallel β-sheet (β 1-6/7) which is used for DNA binding,located between an N-terminal helix and a short helix [7,8]. Most importantly, Val119-Ser183, lys123 and lys126, along with Lys79, Arg85,and Arg 88 were identified as biochemically crucial for DNA binding. Arg88 is conserved in all NAC proteins but Lys79 and Arg85 could be exchangable but exert different DNA binding affinity [9]. The NAC domain
1.Kubo et al (2005) Transcription swtiches for protoxylem and metaxylem vessel formation. Gene Dev.16, 1855-1860.
2.Wang et al (2011) On-Off switches for secondary cell wall biosynthesis. Mol plant. doi:10.1093/mp/ssr098
3.Puranik et al. (2012) NAC proteins: regulation and role in stress tolerance. doi:10.1016/j.tplants.2012.2.14
4.Shen et al (2009)A bioinformatic analysis of NAC genes for plant cell wall development in relation to lignocellulosic bioenergy production. Bioener.Res. 2,217-232.
5.Olsen et al (2005) NAC transcription factor : structurally distinct, functionally diverse. Trends Plant Sci. 10,79-87
6.Ernst et al (2004)Structure of the conserved domain of ANAC,a member of the NAC family of transcription factors. EMBO J 5,297-303
7.Chen et al(2011)A structual view of the conserved domain of rice stress-responsive NAC1. Protein cell 2, 55-63
8.Puranik et al. (2011) Molecular cloning and characterization of a membrane associated NAC family gene, SiNAC from foxtail millet. Mol. Biotech.49,138-150.
9.Tran,L.S.P et al. (2009) Molecular characterization of stress-inducable GmNAC genes in soybean.Mol. Genet.Genomics 281.647-664.