1ndg: Difference between revisions
New page: left|200px<br /> <applet load="1ndg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ndg, resolution 1.90Å" /> '''Crystal structure o... |
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[[Image:1ndg. | [[Image:1ndg.jpg|left|200px]]<br /><applet load="1ndg" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="1ndg, resolution 1.90Å" /> | caption="1ndg, resolution 1.90Å" /> | ||
'''Crystal structure of Fab fragment of antibody HyHEL-8 complexed with its antigen lysozyme'''<br /> | '''Crystal structure of Fab fragment of antibody HyHEL-8 complexed with its antigen lysozyme'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1NDG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ACY as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | 1NDG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NDG OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: antibody; lysozyme; mutant; hyhel-8]] | [[Category: antibody; lysozyme; mutant; hyhel-8]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:28:38 2008'' | ||
Revision as of 17:28, 15 February 2008
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Crystal structure of Fab fragment of antibody HyHEL-8 complexed with its antigen lysozyme
OverviewOverview
The process whereby the immune system generates antibodies of higher, affinities during a response to antigen (affinity maturation) is a, prototypical example of molecular evolution. Earlier studies have been, confined to antibodies specific for small molecules (haptens) rather than, for proteins. We compare the structures of four antibodies bound to the, same site on hen egg white lysozyme (HEL) at different stages of affinity, maturation. These X-ray snapshots reveal that binding is enhanced, not, through the formation of additional hydrogen bonds or van der Waals, contacts or by an increase in total buried surface, but by burial of, increasing amounts of apolar surface at the expense of polar surface, accompanied by improved shape complementarity. The increase in hydrophobic, interactions results from highly correlated rearrangements in antibody, residues at the interface periphery, adjacent to the central energetic hot, spot. This first visualization of the maturation of antibodies to protein, provides insights into the evolution of high affinity in other, protein-protein interfaces.
About this StructureAbout this Structure
1NDG is a Protein complex structure of sequences from Gallus gallus and Mus musculus with as ligand. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
ReferenceReference
X-ray snapshots of the maturation of an antibody response to a protein antigen., Li Y, Li H, Yang F, Smith-Gill SJ, Mariuzza RA, Nat Struct Biol. 2003 Jun;10(6):482-8. PMID:12740607
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