1lzi: Difference between revisions
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'''Glycosyltransferase A + UDP + H antigen acceptor'''<br /> | '''Glycosyltransferase A + UDP + H antigen acceptor'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1LZI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with HG, MN and UDP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glycoprotein-fucosylgalactoside_alpha-N-acetylgalactosaminyltransferase Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.40 2.4.1.40] Full crystallographic information is available from [http:// | 1LZI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=HG:'>HG</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=UDP:'>UDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glycoprotein-fucosylgalactoside_alpha-N-acetylgalactosaminyltransferase Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.40 2.4.1.40] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LZI OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transmembrane]] | [[Category: transmembrane]] | ||
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Revision as of 17:21, 15 February 2008
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Glycosyltransferase A + UDP + H antigen acceptor
OverviewOverview
The human ABO(H) blood group antigens are produced by specific, glycosyltransferase enzymes. An N-acetylgalactosaminyltransferase (GTA), uses a UDP-GalNAc donor to convert the H-antigen acceptor to the A, antigen, whereas a galactosyltransferase (GTB) uses a UDP-galactose donor, to convert the H-antigen acceptor to the B antigen. GTA and GTB differ, only in the identity of four critical amino acid residues. Crystal, structures at 1.8-1.32 A resolution of the GTA and GTB enzymes both free, and in complex with disaccharide H-antigen acceptor and UDP reveal the, basis for donor and acceptor specificity and show that only two of the, critical amino acid residues are positioned to contact donor or acceptor, substrates. Given the need for stringent stereo- and regioselectivity in, this biosynthesis, these structures further demonstrate that the ability, of the two enzymes to distinguish between the A and B donors is largely, determined by a single amino acid residue.
DiseaseDisease
Known disease associated with this structure: Blood group, ABO system OMIM:[110300]
About this StructureAbout this Structure
1LZI is a Single protein structure of sequence from Homo sapiens with , and as ligands. Active as Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, with EC number 2.4.1.40 Full crystallographic information is available from OCA.
ReferenceReference
The structural basis for specificity in human ABO(H) blood group biosynthesis., Patenaude SI, Seto NO, Borisova SN, Szpacenko A, Marcus SL, Palcic MM, Evans SV, Nat Struct Biol. 2002 Sep;9(9):685-90. PMID:12198488
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